葡萄座腔菌Botryosphaeria dothidea胞内miRNA及siRNA的鉴定与分析  

Identification and Analysis of miRNA and siRNA in Botryos phaeria dothidea

作　　者：章鹏程[1] 俞沁如 刘瑶瑶 王寒怡 胡奕然 赖童飞[1] 周婷[1] ZHANG Pengcheng;YU Qinru;LIU Yaoyao;WANG Hanyi;HU Yiran;LAI Tongfei;ZHOU Ting(College of Life and Environmental Sciences,Hangzhou Normal University,Hangzhou 311121,China)

机构地区：[1]杭州师范大学生命与环境科学学院,浙江杭州311121

出　　处：《杭州师范大学学报（自然科学版）》2023年第2期158-166,共9页Journal of Hangzhou Normal University(Natural Science Edition)

基　　金：浙江省教育厅科研项目(Y202044822);浙江省自然科学基金项目(LY22C150009).

摘　　要：通过ITS-rDNA检测和形态学典型特征观察确定葡萄座腔菌Botryosphaeria dothidea的遗传背景,并对其胞内小RNA测序.新预测10个miRNAs和298个siRNAs,并分析了预测的miRNAs和siRNAs的序列信息、结构、长度分布、碱基偏好性、表达情况,为在分子水平了解葡萄座腔菌发育和致病机制提供了理论基础.The genetic background of Botryosphaeria dothidea was confirmed by internal transcribed spacer-ribosomal DNA(ITS-rDNA)detection and morphological characteristics observation.Then,small RNAs in Botryosphaeria dothidea were analyzed.A total of 10 novel miRNAs and 298 siRNAs were predicted.The sequence information,construction,length distribution,base bias and expression of these predicted miRNAs and siRNAs were also analyzed.The results could provide a theoretical foundation for understanding development and pathogenicity mechanisms of Botryosphaeria dothidea at the molecular level.

关 键 词：葡萄座腔菌 内转录间隔区 微小RNA 小干扰RNA 

分 类 号：S436.5[农业科学—农业昆虫与害虫防治]