微生物共培养产生新颖活性次生代谢产物的研究进展  

Advances in producing novel active secondary metabolites by co-culture of microorganisms

作　　者：李洪涛 周皓[1] 丁中涛[1,3] LI Hong-tao;ZHOU Hao;DING Zhong-tao(School of Chemical Science and Technology,Yunnan University,Kunming 650091,Yunnan,China;School of Pharmaceutical Science,Kunming Medical University,Kunming 650500,Yunnan,China;School of Traditional Chinese Medicine,Yunnan University of Chinese Medicine,Kunming 650500,Yunnan,China)

机构地区：[1]云南大学化学科学与工程学院,云南昆明650091 [2]昆明医科大学药学院,云南昆明650500 [3]云南中医药大学中药学院,云南昆明650500

出　　处：《云南大学学报（自然科学版）》2023年第2期493-512,共20页Journal of Yunnan University(Natural Sciences Edition)

基　　金：国家自然科学基金(81860623);云南省科技厅创新研究团队计划(202105AE160006);云南省科技厅项目(202101AT070629,202101AY070001-246).

摘　　要：微生物源天然产物是药物先导化合物的重要资源之一.近年来,利用微生物共培养产生各类天然产物的研究发展迅速.微生物共培养是一种通过模拟(微生物)栖息地/栖息环境以激活可能的沉默生物合成基因簇并进而诱导微生物产生新次生代谢物的有效方法.与菌株纯培养相比,共培养不仅增加了次级代谢产物的种类多样性,而且其生物活性及产量也得到了提高.文章就近5年来真菌与真菌、真菌与细菌、细菌与细菌等3种类型的微生物共培养研究成果进行了综述,同时简要介绍了用于分析共培养作用机制的多组学技术,并在此基础上探讨了微生物共培养相互作用机制研究的进展及挑战.综述旨在为微生物共培养的深入研究提供借鉴,并为微生物药物的开发提供新思路.Natural products derived from microorganisms are one of the important sources of drug lead compounds.In recent years,the research of microbial co-culture to produce various natural products has developed rapidly.Microbial co-culture is an effective method to induce microbial production of novel secondary metabolites by simulating habitats that may activate gene clusters for cryptic secondary metabolite biosynthesis.Compared with the single strain fermentation,co-culture increased the chemical diversity of secondary metabolites and improved the biological activity or yield of secondary metabolites.This paper reviewed the recent results of microbial co-culture research from 2018−2022.Moreover,the multi-omics techniques for analyzing the interaction mechanism of microbial co culture were briefly introduced.This review aims to provide reference for further research of microbial co-culture and new ideas for microbial medicine development.

关 键 词：微生物 共培养 次生代谢产物 生物活性 相互作用机制 

分 类 号：Q939.9[生物学—微生物学]