土壤水分胁迫对马铃薯块茎淀粉合成关键酶基因表达及酶活性的影响  

Effects of soil water stress on gene expression and activity of starch synthesis key enzymes in potato tuber

作　　者：潘念 苏旺 周云[1] 王舰[1] PAN Nian;SU Wang;ZHOU Yun;WANG Jian(Academy of Agriculture and Forestry Sciences,Qinghai University,Qinghai Academy of Agriculture and Forestry Sciences,State Key Laboratory of Plateau Ecology and Agriculture,Key Laboratory of Qinghai-Tibet Plateau Biotechnology of Ministry of Education,Northwest Potato Engineering Research Center of Ministry of Education,Qinghai University,Key Laboratory of Potato Breeding of Qinghai Province,Xining,Qinghai 810016,China)

机构地区：[1]青海大学农林科学院/青海省农林科学院,青海大学省部共建三江源生态与高原农牧业国家重点实验室,青海大学青藏高原生物技术教育部重点实验室,青海大学西北马铃薯教育部工程研究中心,青海省马铃薯育种重点实验室,青海西宁810016

出　　处：《干旱地区农业研究》2023年第2期78-85,共8页Agricultural Research in the Arid Areas

基　　金：青海省创新平台建设专项(2021-ZJ-Y09);财政部和农业农村部:国家现代农业产业技术体系(CARS-09)。

摘　　要：为探讨马铃薯块茎淀粉合成对土壤水分胁迫的响应机制,以马铃薯品种‘青薯9号’和‘闽薯1号’为材料,采用盆栽试验,设置3个水分胁迫处理:正常供水(保持土壤田间持水量的75%)、中度水分胁迫(保持土壤田间持水量的50%)、重度水分胁迫(保持土壤田间持水量的25%),研究了土壤水分胁迫对马铃薯块茎淀粉合成关键酶基因表达及酶活性的影响。结果表明:在块茎形成过程中,土壤水分胁迫显著提高了马铃薯块茎AGPase、GBSSI、SBEI、SBEII、SSII、SSIII基因表达量,随土壤水分胁迫程度的提高,马铃薯块茎SBEI、SBEII、SSII基因表达量上调;土壤水分胁迫显著降低了马铃薯块茎AGPase、GBSS、SBE、SSS酶活性,其中GBSS、SBE酶活性下调;而AGPase、GBSSI、SSIII基因表达量及AGPase、SSS酶活性在马铃薯青薯9号、闽薯1号品种间表现出相反的上、下调趋势。To explore the response mechanism of potato tuber starch synthesis to soil water stress,three water stress treatments of normal water supply(maintaining 75%of soil field water holdings),moderate water stress(maintaining 50%soil field holdings)and severe water stress(maintaining 25%of soil water content)were set up by pot experiments using potato varieties‘Green Potato No.9’and‘Min Potato No.1’.The effect of soil water stress on expression of key enzyme genes and enzyme activity in potato tuber starch synthesis was studied by 50%of water stress and severe water stress(maintaining 25%of soil water content).The results showed that during tuber formation,soil water stress significantly improved the expression of AGPase,GBSSI,SBEI,SBEII,SSII and SSIII genes in potato tubers,and the degree of soil moisture stress,the SBEI,SBEII and SSII gene expression were upregulated.Soil water stress significantly reduced AGPase,GBSS,SBE,SSS activities in potato tubers,among which GBSS and SBE enzyme activity were down regulated.However,AGPase,GBSSI,SSIII gene expression and AGPase and SSS activity showed an opposite upward and downward trend between potato varieties‘Green Potato No.9’and‘Min Potato No.1’.

关 键 词：马铃薯块茎 土壤水分胁迫 淀粉合成关键酶 基因表达 酶活性 

分 类 号：S532[农业科学—作物学] Q945.78[生物学—植物学]