清原马鹿新型人工输精技术的研究  

Research of new artificial insemination technique for Qingyuan wapiti

作　　者：刘贺[1] 赵刚 张博 刘景玉 王哲 李寅博[1] 那飞 崔雨葳 Liu He;Zhao Gang;Zhang Bo;Liu Jingyu;Wang Zhe;Li Yinbo;Na Fei;Cui Yuwei(Centre for Modern Agriculture,Poverty Alleviation and Development in Fushun City,Liaoning Fushun 113006;Centre for Agricultural Development Service in Liaoning Province,Liaoning Shengyang 110031;Centre for Agricultural Development Service in Fushun County,Liaoning Fushun 113008)

机构地区：[1]抚顺市动物疫病预防控制中心,辽宁抚顺113006 [2]辽宁省农业发展服务中心,辽宁沈阳110031 [3]抚顺县农业发展服务中心,辽宁抚顺113008

出　　处：《现代畜牧兽医》2023年第3期7-10,共4页Modern Journal of Animal Husbandry and Veterinary Medicine

基　　金：抚顺市“抚顺英才计划”项目资助(FSYC202104002)。

摘　　要：试验旨在通过建立动物模型创新清原马鹿人工输精方法,有效提高冻精的利用率、确保较高受胎率。试验采用“半剂量”方法制作清原马鹿冻精,研究不同输精次数、剂量以及输精保定方式对受胎率的影响。结果显示:半支剂量组与1支剂量组解冻后的平均精子活力差异不显著(P>0.05)。当两种发情方式均采用单次输精法时,1支组的人工输精受胎率均极显著高于半支组(P<0.01);当两种发情方式均采用二次输精法时,采用“半支+半支”“1支+半支”“1支+1支”方式的人工输精受胎率均接近90%,但差异不显著(P>0.05)。机械保定组的受胎率略高于麻醉保定组,但差异不显著(P>0.05)。研究表明,试验创制的清原马鹿人工输精半支剂量的冻精细管符合人工输精的标准,二次输精法和“半支+半支”的输精剂量是清原马鹿人工输精时的最佳输精方法和输精剂量;机械保定比麻醉保定更适用于人工输精。The aim of the experiment was to establish an animal model to innovate the artificial insemination method of Qingyuan red deer, effectively improve the utilization rate of frozen semen and ensure a higher conception rate. In this experiment, ’half dose’ method was used to prepare frozen semen of Qingyuan red deer, and the effects of different times,doses and methods of semen transfusion on the conception rate were studied. The results showed as follows there was no significant difference in mean sperm motility between half dose group and one dose group after thawing(P>0.05). When single sperm transfusion method was used in both estrus modes, the rate of conception in one group was extremely higher than that in half group(P<0.01). When the two oestrus methods were used twice, the fertilization rates of artificial insemination using ’half branch + half branch’ ’one branch + half branch’ and ’one branch +one branch’ were close to 90%,but the difference was not significant(P>0.05). The conception rate of mechanical preservation group was slightly higher than that of anesthesia preservation group, but the difference was not significant(P>0.05). The study indicated that the frozen spermatoses with half dose of artificial spermatoses in Qingyuan red deer were in line with the standard of artificial spermatoses. The method of secondary spermatoses and the dose of ’one branch +one branch’ were the best methods and doses for artificial spermatoses in Qingyuan red deer. Mechanical guarantee is more suitable for artificial insemination than anesthetic guarantee.

关 键 词：清原马鹿 人工输精 冻精剂量 输精次数 输精剂量 保定方式 

分 类 号：S814[农业科学—畜牧学]