枯草芽胞杆菌GB519在水稻植株中的定殖及对稻瘟病田间防效  

Colonization dynamics of Bacillus subtilis GB519 in rice plants and its biocontrol efficacy against rice blast in the field

作　　者：祁山颜 朱峰[2] 王继春[2] 田成丽 王东元 欧玉苹 刘晓梅[2] 李莉[2] 姜兆远[2] QI Shanyan;ZHU Feng;WANG Jichun;TIAN Chengli;WANG Dongyuan;OU Yuping;LIU Xiaomei;LI Li;JIANG Zhaoyuan(College of Plant Protection,Jilin Agricultural University,Changchun 130118,China;Institute of Plant Protection,Key Laboratory of Integrated Management on Crop Pests in Northeast,Ministry of Agriculture and Rural Affairs,Jilin Academy of Agricultural Sciences,Gongzhuling 136100,China)

机构地区：[1]吉林农业大学植物保护学院,长春130118 [2]吉林省农业科学院植物保护研究所,农业农村部东北作物有害生物综合治理重点实验室,公主岭136100

出　　处：《植物保护》2023年第2期48-56,共9页Plant Protection

基　　金：吉林省农业科技创新工程(CXGC2021TD001,CXGC2021ZY018)。

摘　　要：枯草芽胞杆菌GB519是一株具有广谱抑菌活性的生防菌株。本研究利用绿色荧光蛋白标记的菌株GB519-GFP处理水稻种子、根和叶片,结合激光共聚焦显微镜观察和抗生素平板回收检测的方法,探究其在水稻根茎叶中的定殖动态。结果显示:经GB519-GFP发酵液处理水稻种子、根和叶片后,菌株均可内生定殖于植株的表皮、皮层和维管束中,表明其可在水稻植株内迁移和定殖。GB519-GFP在处理部位的定殖量通常呈现先减少后增多的趋势,非处理部位3~5 d后即可检测到标记菌株。浸种处理,3 d后在幼芽中可检测到标记菌株;20 d后在根中的菌量最多,达5.7×10^(5) cfu/g。灌根处理,1 d后根中菌量为5.4×10^(5) cfu/g;20 d后根、茎和叶中菌量均达到最大值;处理80 d后,根中定殖数量仍达1.9×10^(5) cfu/g。叶面喷施处理,1 d后叶片菌量为4.2×10^(5) cfu/g;20 d后叶片菌量达4.4×10^(5) cfu/g。不同处理方法在各部位的定殖量几乎均在处理20 d后达到峰值。采用叶面喷施GB519对稻瘟病穗颈瘟的田间防效达73.9%,表明叶面喷施GB519能够起到防控稻瘟病的作用。本研究为使用枯草芽胞杆菌GB519防控水稻稻瘟病提供指导。Bacillus subtilis GB519 displays antimicrobial activity in a broad spectrum.In this study,the GFP-labeled strain,GB519-GFP was used to explore its colonization dynamics in rice plants via both the laser confocal microscope observation and antibiotic plate recovery.The strain GB519-GFP could colonize the epidermis,cortex and vascular bundle after seed-soaking,root-irrigating or foliar-spray with the fermentation broth.These results indicated that it could migrate and colonize in rice plants.In general,the colonization quantity of GB519-GFP first decreased and then increased in the treated part,and it could be detected in the untreated part after 3-5 days.The GFP-labeled strain was detected in the bud three days after seed-soaking and the maximum amount of colonization reached 5.7×10^(5) cfu/g in the root after 20 d.The amount of colonization in the root was 5.4×10^(5) cfu/g one day after root-irrigating.The amount reached the maximum value in the root,stem and leaf after 20 d,and remained at 1.9×10^(5) cfu/g in the root after 80 d.The colonization was 4.2×10^(5) cfu/g in the leaves one day after foliar-spray,and reached 4.4×10^(5) cfu/g after 20 d.The amounts of colonization in different tissues reached the peak 20 d after treated.The control efficacy of GB519 against rice blast was 73.9%in the field by foliar-spray.It indicated that foliar spraying with GB519 could play a role in preventing rice blast.This study provides a guidance for the control of rice blast by B.subtilis GB519.

关 键 词：枯草芽胞杆菌GB519 GFP 定殖动态 稻瘟病 防效 

分 类 号：S476[农业科学—农业昆虫与害虫防治]