多花黄精炭疽病病原菌鉴定  

Identification of pathogen causing anthracnose on Polygonatum cyrtonema Hua

作　　者：但雨柔 刘铭 崔馨燕 马万里 汤子萱 尹福强 DAN Yurou;LIU Ming;CUI Xinyan;MA Wanli;TANG Zixuan;YIN Fuqiang(College of Biological and Food Engineering,Chongqing Three Gorges University,Chongqing 404120,China;Chongqing Engineering Laboratory for Green Cultivation and Deep Processing of the Three Gorges Reservoir Area’s Medicinal Herbs,Chongqing 404120,China)

机构地区：[1]重庆三峡学院生物与食品工程学院,重庆404120 [2]三峡库区道地药材绿色种植与深加工重庆市工程实验室,重庆404120

出　　处：《植物保护》2023年第2期288-293,共6页Plant Protection

基　　金：重庆市技术创新与应用发展专项重点项目(cstc2020jscx-tpyzxX0007)。

摘　　要：炭疽病是多花黄精最严重的病害之一。本研究从重庆市万州区采集疑似为炭疽病的多花黄精病叶,分离培养获得了培养性状不同的2株炭疽菌,致病性测定表明这2株菌株都可引起多花黄精炭疽病;利用核糖体内转录间隔区(rDNA-ITS)、肌动蛋白(ACT)、几丁质合成酶(CHS-1)和β-微管蛋白(TUB)基因进行多基因系统发育分析,发现这2株菌株分别与松针炭疽菌Colletotrichum fioriniae和博宁炭疽菌C.boninense聚在一起,形成明显分支。结合形态学特点,确定引起多花黄精炭疽病的病原菌为松针炭疽菌C.fioriniae和博宁炭疽菌C.boninense。其中松针炭疽菌C.fioriniae引起黄精炭疽病是首次报道。Anthracnose is one of the most serious diseases on Polygonatum cyrtonema.Two strains were isolated from leave samples with symptoms of anthracnose of P.cyrtonema collected from Wanzhou,Chongqing.Following infection assay on the plant suggested that both strains were the pathogenic agents that were identified as Colletotrichum fioriniae and C.boninense based on morphological,cultural characteristics and phylogenetic analyses of internal transcribed space(ITS),actin(ACT),chitin synthase 1(CHS-1)andβ-tubulin(TUB)gene.And this is the first description of C.fioriniae pathogen responsible for P.cyrtonema anthracnose.

关 键 词：多花黄精 分离鉴定 多基因系统发育分析 松针炭疽菌 博宁炭疽菌 

分 类 号：S432.1[农业科学—植物病理学]