生防菌在棉田土壤中定殖数量与防病作用相关性  

The Quantity and Activity of Biocontrol Bacteria in Cotton Field Soil and the Correlation of Disease Prevention

作　　者：王静怡 佐长赓 牛新湘[4,5] 杨红梅 楚敏[2,3] 王宁 林青[2,3] 王有武 娄恺[2,3] 史应武 WANG Jingyi;ZUO Changgeng;NIU Xinxiang;YANG Hongmei;CHU Min;WANG Ning;LIN Qing;WANG Youwu;LOU Kai;SHI Yingwu(College of Life Sciences and Technology,Xinjiang University,Urumqi 830052,China;Research Institute of Applied Microbiology,Xinjiang Academy of Agricultural Sciences,Urumqi 830091,China;Xinjiang Special Environmental Microbiology Laboratory,Urumqi 830091,China;Institute of Soil and Fertilizer and Agricultural Water Conservation,Xinjiang Academy of Agricultural Sciences,Urumqi 830091,China;Key Laboratory of Northwest Oasis Agricultural Environment,Ministry of Agriculture and Rural Affairs,Urumqi 830091,China)

机构地区：[1]新疆大学生命科学与技术学院,乌鲁木齐830052 [2]新疆农业科学院微生物应用研究所,乌鲁木齐830091 [3]新疆特殊环境微生物实验室,乌鲁木齐830091 [4]新疆农业科学院土壤肥料与农业节水研究所,乌鲁木齐830091 [5]农业农村部西北绿洲农业环境重点实验室,乌鲁木齐830091

出　　处：《新疆农业科学》2023年第1期178-184,共7页Xinjiang Agricultural Sciences

基　　金：国家自然科学基金(31860024,41471220);新疆维吾尔自治区自然科学基金面上项目(2018D01A44);国家重点研发计划(2017YFD0200600);新疆农业科学院青年科技骨干创新能力培养项目(xjnkq-2019014);自治区科技计划重大专项(2020A01002);新疆农业科学院农业科技创新平台能力提升建设专项(nkypt005)。

摘　　要：【目的】研究拮抗菌施入棉田土壤后生防菌的数量变化和棉花黄萎病的发病情况,分析生防菌的定殖与防病的相互关系,为生防细菌在棉花黄萎病的防治应用提供理论依据。【方法】选择4株棉花内生和根际拮抗菌Bacillus atrophaeus SHZ-24、Bacillus vanillea SMT-24、Bacillu subtilis SHT-15和Bacillus velezensis BHZ-29进行盆栽试验,使用平板计数和实时荧光定量分析细菌和病原菌。【结果】4株拮抗细菌在土壤中数量变化趋势相同,50 d时的存活量从高到低分别为SHZ-24、BHZ-29、SMT-24、SHT-15,4株拮抗细菌处理均可降低棉苗的病情指数。【结论】BHZ-29的防病效果较好。4株拮抗细菌在土壤中的定殖数量与防病效果呈显著正相关。【Objective】Verticillium dahliae is a soil-borne disease caused by Verticillium dahliae colonizing vascular bundles by infecting roots.Biological control is the most promising control method.Endophytic and rhizosphere bacteria are considered to be the most potential microbial flora in biocontrol bacteria.This project aims to study the changes in the number of biocontrol bacteria and the incidence of cotton Verticillium wilt after antagonistic bacteria is applied to cotton soil,and explore the relationship between the colonization of biocontrol bacteria and disease prevention so as to provide the application of biocontrol bacteria in the control of cotton Verticillium,hoping this theoretical basis will provide technical support for biological control to ensure the quality of agricultural products and safe production.【Methods】In this study,4 cotton plants were selected for pot experiment with endophytic and rhizosphere antagonistic bacteria Bacillus atrophaeus SHZ-24,Bacillus vanillea SMT-24,Bacillu subtilis SHT-15 and Bacillus velezensis BHZ-29,using plate counting and real-time fluorescence quantification quantitative analysis of bacteria and pathogens.【Results】The number of 4 antagonistic bacteria in the soil changed in the same trend,and the survival rate at 50 days was SHZ-24,BHZ-29,SMT-24,SHT-15 from high to low,and the 4 antagonistic bacteria could be reduced by treatment.All four antagonistic bacterial treatments could reduce the disease index of cotton seedlings.【Conclusion】BHZ-29 worked better.The colonization of the 4 antagonistic bacteria in the soil is significantly positively correlated with the disease prevention effect.

关 键 词：拮抗细菌 大丽轮枝菌 实时荧光定量 定殖数量 防病作用 

分 类 号：S188[农业科学—农业基础科学]