BnbHLH92a negatively regulates anthocyanin and proanthocyanidin biosynthesis in Brassica napus  

作　　者：Ran Hu Meichen Zhu Si Chen Chengxiang Li Qianwei Zhang Lei Gao Xueqin Liu Shulin Shen Fuyou Fu Xinfu Xu Ying Liang Liezhao Liu Kun Lu Hao Yu Jiana Li Cunmin Qu 

机构地区：[1]Chongqing Rapeseed Engineering Research Center,College of Agronomy and Biotechnology,Southwest University,Chongqing 400715,China [2]Academy of Agricultural Sciences,Southwest University,Chongqing 400715,China [3]Engineering Research Center of South Upland Agriculture,Ministry of Education,Chongqing 400715,China [4]Department of Biological Sciences,Faculty of Science,National University of Singapore,Singapore 117558,Singapore [5]Agriculture and Agri-Food Canada,Saskatoon Research Centre,107 Science Place,Saskatoon,Saskatchewan S7N 02X,Canada [6]Temasek Life Sciences Laboratory,National University of Singapore,Singapore 117604,Singapore

出　　处：《The Crop Journal》2023年第2期374-385,共12页作物学报（英文版）

基　　金：supported by the National Natural Science Foundation of China(32072093,31830067);the China Agriculture Research System of MOF and MARA,the Science and Enterprise Consortium Project of Chongqing(cqnyncw-kqlhtxm);the Innovation and Entrepreneurship Training Program for Undergraduates(S202010635197);the 111 Project(B12006).

摘　　要：Yellow seed trait is a desirable characteristic with potential for increasing seed quality and commercial value in rapeseed,and anthocyanin and proanthocyanidins(PAs)are major seed-coat pigments.Few transcription factors involved in the regulation of anthocyanin and PAs biosynthesis have been characterized in rapeseed.In this study,we identified a transcription factor gene BnbHLH92a(BnaA06T0441000ZS)in rapeseed.Overexpressing BnbHLH92a both in Arabidopsis and in rapeseed reduced levels of anthocyanin and PAs.Correspondingly,the expression profiles of anthocyanin and PA biosynthesis genes(TT3,BAN,TT8,TT18,and TTG1)were shown by quantitative real-time PCR to be inhibited in BnbHLH92a-overexpressing Arabidopsis seeds,indicating that BnbHLH92a represses the anthocyanin and PA biosynthesis pathway in Arabidopsis.BnbHLH92a physically interacts with the BnTTG1 protein and represses the biosynthesis of anthocyanins and PAs in rapeseed.BnbHLH92a also binds directly to the BnTT18 promoter and represses its expression.These results suggest that BnbHLH92a is a novel upstream regulator of flavonoid biosynthesis in B.napus.

关 键 词：Brassica napus L BnbHLH92a ANTHOCYANIN PROANTHOCYANIDINS Flavonoid pathway 

分 类 号：S565.4[农业科学—作物学]