Identification of KASP markers and putative genes for pre-harvest sprouting resistance in common wheat(Triticum aestivum L.)  

作　　者：Guannan Liu Daniel Mullan Aimin Zhang Hui Liu Dongcheng Liu Guijun Yan 

机构地区：[1]UWA,School of Agriculture and Environment and The UWA Institute of Agriculture,The University of Western Australia,Perth,WA 6009,Australia [2]InterGrain Pty.Ltd.,19 Ambitious Link Bibra Lake,WA 6163,Australia [3]State Key Laboratory of North China Crop Improvement and Regulation,College of Agronomy,Hebei Agriculture University,Baoding 071000,Hebei,China

出　　处：《The Crop Journal》2023年第2期549-557,共9页作物学报（英文版）

基　　金：funded by Global Innovation Linkage program (GIL53853) from Australian Department of Industry, Science, Energy and Resources;Australian Government RTP Scholarship (International);University Postgraduate Awards (UPA)

摘　　要：Common wheat(Triticum aestivum L.)is the most important crop in the world and a typical allopolyploid with a large and complex genome.Pre-harvest sprouting(PHS)leads to a significant reduction in grain quality worldwide.PHS is a complex trait with related QTL located on different chromosomes.However,the study of markers and genes related to PHS resistance is limited especially for whitegrained wheat.Four pairs of near isogenic lines(NILs)from a white-grained wheat cross of Chara
DM5637B*8 targeting a major QTL for PHS resistance(Qphs.ccsu-3A.1)on wheat chromosme 3AL were genotyped using the 90K SNP Illumina iSelect array.Ten SNPs were identified,with a 75%-100%consistency between genotype and phenotype in the resistant or susceptible isolines.The 10 SNPs were converted to cost-effective kompetitive allele-specific PCR(KASP)markers.Screening of 48 wheat cultivars with different phenotypes of PHS identified four KASP markers with 81.3%-85.4%conformity between genotype and phenotype.Further investigation revealed that the four SNPs(BS00022245_51,Kukri_c49927_151,BS00022884_51 and BS00110550_51)corresponding to the four validated KASP markers are residing in three independent genes(TraesCS3A03G1072800,TraesCS3A03G1072400,TraesCS3A03G1071800)close to each other with a distance of 4.28-4.48 Mb to the targeted QTL.These three annotated genes have potential functions related to PHS resistance.Our study revealed that combined use of NILs and the 90K SNP chip is a powerful approach for developing KASP markers and mining functional genes in wheat.The KASP markers for PHS resistance on chromosome 3AL are useful for high-throughput evaluation and marker-assisted selection,and the three identified genes could lead to a better understanding of the genetic pathways controlling PHS.

关 键 词：Pre-harvest sprouting(PHS) KASP marker Functional genes Chromosome 3AL 90K SNP assay Near-isogenic lines 

分 类 号：S512.1[农业科学—作物学]