Identification and validation of major QTL for grain size and weight in bread wheat(Triticum aestivum L.)  

作　　者：Guangsi Ji Zhibin Xu Xiaoli Fan Qiang Zhou Liangen Chen Qin Yu Simin Liao Cheng Jiang Bo Feng Tao Wang 

机构地区：[1]Chengdu Institute of Biology,Chinese Academy of Sciences,Chengdu 610041,Sichuan,China [2]University of Chinese Academy of Sciences,Beijing 100049,China [3]The Innovative Academy of Seed Design,Chinese Academy of Sciences,Beijing 100101,China

出　　处：《The Crop Journal》2023年第2期564-572,共9页作物学报（英文版）

基　　金：supported by the Strategic Priority Research Program of Chinese Academy of Sciences(XDA24030402);Sichuan Science and Technology Program.

摘　　要：Grain size and weight are key components of wheat yield.Exploitation of major underlying quantitative trait loci(QTL)can improve yield potential in wheat breeding.A recombinant inbred line(RIL)population was constructed to detect QTL for thousand-grain weight(TGW),grain length(GL)and grain width(GW)across eight environments.Genomic regions associated with grain size and grain weight were identified on chromosomes 4A and 6A using bulked segregant exome sequencing(BSE-Seq)analysis.After constructing genetic maps,six major QTL detected in at least four individual environments and in best linear unbiased estimator(BLUE)datasets,explained 7.50%-23.45%of the phenotypic variation.Except for QGl.cib-4A,the other five QTL were co-located in two regions,namely QTgw/Gw.cib-4A and QTgw/Gw/Gl.cib-6A.Interactions of these QTL were analyzed.Unlike QTgw/Gw/Gl.cib-6A,QTgw/Gw.cib-4A and QGl.cib-4A had no effect on grain number per spike(GNS).The QTL were validated in a second cross using Kompetitive Allele Specific PCR(KASP)markers.Since QTgw/Gw.cib-4A was probably a novel locus,it and the KASP markers reported here can be used in wheat breeding.TraesCS4A03G0191200 was predicted to be potential candidate gene for QTgw/Gw.cib-4A based on the sequence differences,spatiotemporal expression patterns,gene annotation and haplotype analysis.Our findings will be useful for fine mapping and for marker-assisted selection in wheat grain yield improvement.

关 键 词：BSE-Seq Thousand-grain weight Grain length Grain width QTL mapping 

分 类 号：S512.1[农业科学—作物学]