Molecular cytogenetic analyses of two new wheat-rye 6RL translocation lines with resistance to wheat powdery mildew  

作　　者：Shanying Zhu Haonan Du Fuyu Su Jin Wang Qingfeng Meng Tianlei Liu Rui Guo Zhaozhao Chen Huanhuan Li Wenxuan Liu Pengtao Ma Huagang He 

机构地区：[1]School of Life Sciences,Jiangsu University,Zhenjiang 212013,Jiangsu,China [2]School of Environment and Safety Engineering,Jiangsu University,Zhenjiang 212013,Jiangsu,China [3]College of Life Sciences,Yantai University,Yantai 264005,Shandong,China [4]College of Life Sciences,Henan Agricultural University,Zhengzhou 450002,Henan,China

出　　处：《The Crop Journal》2023年第2期584-592,共9页作物学报（英文版）

基　　金：supported by the National Natural Science Foundation of China(32171990 and 32072053);Key Research and Development Program of Zhenjiang(NY2021001);State Key Laboratory of Plant Cell and Chromosome Engineering(PCCE-KF-2021-05 and PCCE-KF-2022-07);State Key Laboratory of Crop Biology in Shandong Agricultural University(2021KF01);Natural Science Foundation of the Jiangsu Higher Education institutions of China(21KJB210004);Open Project Funding of State Key Laboratory of Crop Stress Adaptation and Improvement(CX1130A0920014);Key Research and Development Program of Shandong Province(2020CXGC010805).

摘　　要：Rye(Secale cereale)is a valuable gene donor for wheat improvement,especially for its resistance to diseases.Developing rye-derived resistance sources is important for wheat breeding.In the present study,two wheat-rye derivatives,designated JS016 and JS110,were produced by crossing common wheat cultivar Yangmai 23 with Pakistani rye accession W2A.Using sequential genomic in situ hybridization(GISH)and multicolor fluorescence in situ hybridization(mc-FISH),JS016 and JS110 were identified as a T6BS.6RL translocation line and a T6BS.6BL6RL translocation line,respectively.Ten newly 6RL chromosome arm-specific markers were developed and used to confirm the 6RL translocation.The wheat 55K single-nucleotide polymorphism(SNP)array further verified the molecular cytogenetic identification results above and clarified their breakpoints at 430.9 and 523.0 Mb of chromosome 6B in JS016 and JS110,respectively.Resistance spectrum and allelism test demonstrated that JS016 and JS110 possessed novel powdery mildew resistance gene(s)that was derived from the 6RL translocation but differed from Pm20.Moreover,JS016 and JS110 had better agronomic traits than the previously reported 6RL translocation line carrying Pm20.To efficiently transfer and detect the 6RL translocation from JS016 and JS110,one 6RL-specific Kompetitive allele specific PCR(KASP)marker was developed and validated in high throughput marker-assisted selection(MAS).

关 键 词：Secale cereale Translocation line Wheat powdery mildew GISH/FISH KASP marker 

分 类 号：S435.121.46[农业科学—农业昆虫与害虫防治]