儿童血管性血友病因子抗原和活性水平参考区间建立  

Establishment of reference intervals of von Willebrand factor antigen and activity for children

作　　者：魏佳 申汉俊 薛建[1] 谭秋霞 WEI Jia;SHEN Hanjun;XUE Jian;TAN Qiuxia(Department of Laboratory Medicine,Chil-dren's Hospital of Soochow University,Suzhou 215025,Jiangsu,China;Blood Transfusion Department,Chil-dren's Hospital of Soochow University,Suzhou 215025,Jiangsu,China)

机构地区：[1]苏州大学附属儿童医院检验科,江苏苏州215025 [2]苏州大学附属儿童医院输血科,江苏苏州215025

出　　处：《临床检验杂志》2023年第1期32-34,共3页Chinese Journal of Clinical Laboratory Science

摘　　要：目的观察性别、年龄和ABO血型对儿童血管性血友病因子(v WF)检测的影响并建立参考区间。方法随机选取2022年1月至9月苏州大学附属儿童医院无出凝血临床表现且常规凝血检查结果均正常的857例儿童,检测其血浆v WF抗原和活性水平与ABO血型。结果≤6岁儿童v WF抗原和活性水平低于>6岁且≤18岁儿童(P<0.05);在不同年龄分组中O型血儿童v WF水平均低于非O型血儿童(P<0.001)。对于≤6岁儿童,O型血儿童v WF抗原和活性参考区间分别为44.4%~130.6%和43.0%~194.7%;非O型血儿童v WF抗原和活性参考区间分别66.3%~190.4%和71.3%~282.3%。对于>6岁儿童,O型血儿童v WF抗原和活性参考区间分别为50.4%~145.4%和51.7%~221.4%;非O型血儿童v WF抗原和活性参考区间分别为75.4%~195.6%和84.0%~296.5%。结论儿童血浆v WF抗原和活性水平受年龄和ABO血型影响,实验室积极建立针对儿童v WF的特异性参考区间有利于临床全面评估患儿的出血与血栓风险。Objective To investigate the effects of gender,age,and ABO blood type on the detection of von Willebrand factor(v WF)antigen(v WF:Ag)and activity(v WF:Ac)in children,and establish the reference intervals of v WF:Ag and v WF:Ac for children.Methods 857 children who had no clinical manifestation of bleeding or coagulation and had normal results of routine coagulation tests were selected randomly from our hospital,and their plasma v WF:Ag and v WF:Ac and ABO blood type were detected.Results The levels of v WF:Ag and v WF:Ac in the low age group(≤6 years old)were significantly lower than that in the high age group(>6 and≤18 years old,P<0.05).The levels of v WF:Ag and v WF:Ac in children with type O blood were significantly lower than that with non-O blood type in different age groups(P<0.001).For children aged≤6 years,the reference intervals of v WF:Ag and v WF:Ac in the type O blood group were 44.4%-130.6%and 43.0%-194.7%,respectively,and those in the non-O blood type group were 66.3%-190.4%and 71.3%-282.3%,respectively.For children aged>6 and≤18 years,the reference intervals of v WF:Ag and v WF:Ac in the type O blood group were 50.4%-145.4%and 51.7%-221.4%,respectively,and those in the non-O blood type were 75.4%-195.6%and 84.0%-296.5%,respectively.Conclusion The levels of plasma v WF:Ag and v WF:Ac in children are affected by age and ABO blood type.Laboratories should actively establish the specific reference intervals of v WF in children,which will be helpful for compre-hensively assessing the risk of bleeding and thrombosis in children.

关 键 词：儿童 血管性血友病因子 ABO血型 参考区间 

分 类 号：R446[医药卫生—诊断学]