八角无性系对炭疽病抗性的快速测定  

Rapid Determination of Anthracnose Resistance of Illicium verum Clones

作　　者：廖旺姣[1] 邹东霞[1] 常明山[1] 钟雅婷[1] 黄乃秀[1] LIAO Wangjiao;ZOU Dongxia;CHANG Mingshan;ZHONG Yating;HUANG Naixiu(Guangxi Zhuang Autonomous Region Forestry Research Institute,Guangxi Key Laboratory of Special Non-timber Forest Cultivation&Utilization,Guangxi Engineering Research Center of Forest Pests Natural Enemies Breeding,Nanning 530002,China)

机构地区：[1]广西壮族自治区林业科学研究院,广西特色经济林培育与利用重点实验室,广西林业有害生物天敌繁育工程技术研究中心,广西南宁530002

出　　处：《云南农业大学学报（自然科学版）》2023年第1期41-46,共6页Journal of Yunnan Agricultural University:Natural Science

基　　金：广西重点研发计划项目(桂科AB1850020)。

摘　　要：[目的]建立一种操作简便、准确可靠的八角无性系炭疽病抗性鉴定方法,为其选育及应用提供理论基础。[方法]采用离体叶片接种法和苗期叶片接种法测定23个八角无性系对炭疽病的抗性水平。[结果]23个八角无性系中,有22个无性系采用2种接种法测定的结果一致,一致性高达95.65%;其中,福进14号和黑叶1号为抗病无性系,福进4号、福进5号和福进6号等9个无性系为中抗无性系,福进2号、福进3号和福进7号等6个无性系为中感无性系,福进8号和福进16号为感病无性系,福进1号、福进11号和福进12号为高感无性系。[结论]2种接种方法均可反应八角无性系抗病性的差异。离体叶片接种法具有操作方便、发病速度快、不伤害植株和耗时短等优点,缺点是鉴定结果可能与实际抗病表现不一致,可以作为八角品系对炭疽病抗性鉴定的重要辅助手段;苗期接种法鉴定结果较为可靠,但缺点是接种后植株落叶严重且难以恢复,需要合适的温、湿度等环境条件。[Purpose]To establish a simple,accurate and reliable disease resistance evaluation system of Illicium verum clones resistant to anthracnose,providing a theoretical basis for the selection and application of I.verum clones.[Methods]The detached leaf inoculation method and the seedling leaf inoculation method were used to determine the resistance level of 23 I.verum clones to anthracnose.[Results]The results of 22 clones of 23 I.verum clones determined by two inoculation methods were consistent,and the consistency was as high as 95.65%.The results showed that Fujin No.14 and Heiye No.1 were disease-resistant clones,nine clones such as Fujin No.4,Fujin No.5 and Fujin No.6 were moderate-resistant clones,six clones including Fujin No.2,Fujin No.3 and Fujin No.7 were moderate-susceptible clones, Fujin No.8 and Fujin No.16 were susceptible clones, Fujin No.1, Fujin No.11 and Fujin No.12 were high-susceptible clones. [Conclusion]Both the two in-oculation methods can reflect the differences in disease resistance of I. verum clones. The detached leaf inoculation method has the advantages of easy operation, fast pathogenesis, no harm to plants and short time-consuming, and its disadvantage is that the results of identification may not be consistent with the actual resistance performance, and it can be used as an important assistant means for the iden-tification of I. verum anthracnose. The identification results of the seedling inoculation method are more reliable, but the disadvantage is that the damage to the plant is too great, the leaves after inocula-tion are serious, the plants are difficult to recover, and appropriate environmental conditions such as temperature and humidity are required.

关 键 词：八角炭疽病 无性系 离体叶片接种法 苗期叶片接种法 抗病性测定 

分 类 号：S763.13[农业科学—森林保护学]