大型迪庆藏猪不同生长阶段腹脂代谢功能基因筛选及其调控网络分析  

Analysis of Functional Genes Screening and Regulatory Networks of Abdominal Fat Metabolism of Large Diqing Tibetan Pigs at Different Growth Stages

作　　者：董新星[1] 王琳 刘金桥 严达伟[1] 张博[2] 邓俊 张浩[2] 马黎[4] DONG Xinxing;WANG Lin;LIU Jinqiao;YAN Dawei;ZHANG Bo;DENG Jun;ZHANG Hao;MA Li(Faculty of Animal Science and Technology,Yunnan Agricultural University,Kunming 650201,China;College of Animal Science and Technology,China Agricultural University,Beijing 100193,China;Yunnan Province Anim-al Husbandry Station,Kunming 650224,China;Department of Animal Husbandry and Veterinary Medicine,Yunnan Vocational and Technical College of Agriculture,Kunming 650212,China)

机构地区：[1]云南农业大学动物科学技术学院,云南昆明650201 [2]中国农业大学动物科学技术学院,北京100193 [3]云南省畜牧总站,云南昆明650224 [4]云南农业职业技术学院畜牧兽医学院,云南昆明650212

出　　处：《云南农业大学学报（自然科学版）》2023年第1期61-70,共10页Journal of Yunnan Agricultural University:Natural Science

基　　金：云南省乡村振兴科技专项(202104BI090021);云南省高校科技创新团队支持计划(云南省高校高原山地畜禽抗逆性基因发掘与利用科技创新团队)。

摘　　要：[目的]筛选大型迪庆藏猪不同生长阶段腹脂代谢差异的功能基因并解析其调控网络。[方法]选择胎次相同、出生日期及体质量相近的大型迪庆藏猪36头,随机分为3组进行育肥试验,分别在体质量达40、80和120 kg屠宰,每组采集3头猪的腹脂进行转录组测序,测序数据经短时间序列表达模式(STEM)趋势分析、功能富集分析和互作网络分析。[结果]40 kg vs 80 kg、80 kg vs 120 kg和40 kg vs 120 kg分别筛选到1517、486和1752个显著差异基因;差异基因STEM分析显示:模块4和1的基因先显著下调后基本不变,模块15、12和11的基因随体质量增加而显著上调,模块0的基因随体质量增加而显著下调;WNT10B、CPT1B和C5AR2位于模块4和1的基因网络核心,PLA2G7、WWTR1、SPP1、SERPINE1和PTPN11位于模块15、12和11的基因网络核心,ADIPOQ、CH25H和IL10位于模块0的基因网络核心;WNT10B和CPT1B等11个基因的qPCR验证结果与转录组测序结果一致。[结论]WNT10B和PTPN11等11个核心基因以协作方式参与大型迪庆藏猪腹膜脂肪代谢调控,结果可为迪庆藏猪的遗传改良提供参考。[Purpose]To screen the key genes regulating abdominal fat(AF)of large Diqing Tibetan pigs(TPs)at different growth stages and analyze their regulatory network.[Method]A total of 36 TPs with the same parity,similar birth date and body weight were selected and randomly divided into three groups for fattening experiments under the same condition.They were slaughtered when their body weight was 40 kg,80 kg,and 120 kg,respectively.AF tissue of three pigs in each group was collected for transcriptome sequencing.The sequencing data were analysed by short-time series expression miner(STEM)analysis,functional enrichment analysis and interaction network ana-lysis.[Result]A total of 1517,486 and 1752 genes were screened at 40 kg vs 80 kg stage,80 kg vs 120 kg stage and 40 kg vs 120 kg stage,respectively.STEM trend analysis of differentially ex-pressed genes showed that the genes of module 4 and module 1 were significantly down-regulated at first and then basically unchanged,the genes of module 15,module 12 and module 11 were signific-antly up-regulated with the increase of body weight,and the genes of module 0 were significantly down-regulated with the increase of body weight.WNT10B,C5AR2 and CPT1B were located at the core of gene interaction network constructed by genes of module 4 and module 1.PLA2G7,WWTR1,SPP1,SERPINE1,and PTPN11 were located at the core of gene interaction network constructed by genes of module 15,module 12,and module 11.ADIPOQ,CH25H,and IL10 were located at the core of gene interaction network constructed by genes of module 0.The qPCR verification results of 11 differential genes including WNT10B and CPT1B were consistent with the transcriptome sequencing results.[Conclusion]A total of 11 core genes,including WNT10B and PTPN11 participated in the regulation of AF metabolism in TPs in a cooperative manner.The results can provide a reference for the genetic improvement of TPs.

关 键 词：迪庆藏猪 腹膜脂肪代谢 RNA-Seq 功能基因 调控网络 

分 类 号：S828.2[农业科学—畜牧学]