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作 者:吴佳妮 邓仕任[1] 陈晓霞[1] 张雯慧 边星 孙璐 夏林波[1] WU Jia-ni;DENG Shi-ren;CHEN Xiao-xia;ZHANG Wen-hui;BIAN Xing;SUN Lu;XIA Lin-bo(College of Pharmacy,Liaoning University of Traditional Chinese Medicine,Dalian Liaoning 116600,China;Key Laboratory of Separation Science for Analytical Chemistry,Chinese Academy of Sciences,Dalian Liaoning 116600,China)
机构地区:[1]辽宁中医药大学药学院,辽宁大连116600 [2]中国科学院分离分析化学重点实验室,辽宁大连116600
出 处:《辽宁化工》2023年第3期450-452,456,共4页Liaoning Chemical Industry
基 金:辽宁省自然科学基金(项目编号:20170540617、2015020713);中国科学院分离分析化学重点实验室开放基金(项目编号:KL2108)。
摘 要:采用UHPLC法建立中药荷丹片中莲碱的含量测定方法。色谱柱为Poroshell 120 EC-C18(3.0 mm×150 mm,2.7μm),流动相为水-乙腈-三乙胺-冰乙酸(68.1∶30∶1.6∶0.3),以0.6 mL·min^(-1)流速等度洗脱,波长272 nm。结果表明:方法的专属性良好,莲碱在0.004~0.04µg范围内的绝对进样量与峰面积间具有良好的线性关系,稳定性、重复性、精密度、加样回收率的RSD均在3%以内。所建立的UHPLC法快速、准确、稳定、可靠,可作为荷丹片中莲碱的含量测定方法。The method to determine the roemerine content in Hedan tablet was established by UHPLC.Poroshell 120 EC-C18(3.0 mm×150 mm,2.7μm)was used as the stationary phase,and the mobile phase was water-acetonitrile-triethylamine-glacial acetic acid(68.1∶30∶1.6∶0.3),equal elution was carried out with the flow rate of 0.6 mL·min^(-1),and the detection wavelength was 272 nm.The results show that,the specificity of the method was good,the absolute sample mass of roemerine from 0.004~0.04µg had a good linear relationship with the peak area.The RSD of stability,repeatability,precision and recovery were within 3%.The established UHPLC method is fast,accurate,stable and reliable,and can be used as a method for measuring roemerine content in Hedan tablet.
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