烯酰吗啉对葡萄叶部代谢的影响  

Effects of dimethomoph on metabolism of grape leaves

作　　者：何涛 孙家秀 陈吉越 邓维萍[1] 何霞红 朱书生[1] 朱有勇[1] 杜飞[1] HE Tao;SUN Jia-xiu;CHEN Ji-yue;DENG Wei-ping;HE Xia-hong;ZHU Shu-sheng;ZHU You-yong;DU Fei(Yunnan Agricultural University/National Engineering Center of Agricultural Biodiversity Application Technology,Kunming,Yunnan 650201,China;Southwest Forestry University,Kunming,Yunnan 650224,China)

机构地区：[1]云南农业大学/农业生物多样性应用技术国家工程研究中心,云南昆明650201 [2]西南林业大学,云南昆明650224

出　　处：《南方农业学报》2022年第12期3433-3443,共11页Journal of Southern Agriculture

基　　金：国家重点研发计划重点专项(2018YFD0201301);国家自然科学基金项目(31860519);云南省中国工程院云南院士工作站项目(2018IC063);云南省教育厅科学研究基金项目(2022Y222)。

摘　　要：【目的】探究羧酸酰胺类杀卵菌剂烯酰吗啉对葡萄叶片代谢物质及代谢通路的影响,为进一步阐释羧酸酰胺类杀菌剂对葡萄叶片代谢的影响提供科学依据。【方法】以避雨栽培未施用过杀菌剂的健康红地球葡萄为研究对象,在葡萄树生长发育期间喷施烯酰吗啉农药处理(DI),以等量无菌水为对照(CK),采用超高效液相色谱—串联质谱(UPLC-MS)技术研究2种不同处理下葡萄叶片的代谢差异。【结果】偏最小二乘回归判别分析及置换检验结果显示2种处理葡萄样品的代谢物存在显著差异(P<0.05,下同),进一步采用正交偏最小二乘判别分析结合置换检验发现2种处理下葡萄叶片有差异代谢物,并筛选出造成2种处理葡萄叶片代谢显著差异的39种主要代谢物质,其中,烯酰吗啉处理后显著改变的差异代谢物质主要分为7大类,脂质和类脂分子物质、苯丙烷和聚酮、有机酸及其衍生物、有机氧化合物、生物碱及其衍生物、有机氮化合物及其他化合物。对植物抗性关键激素水杨酸和茉莉酸,以及与抗性有关的物质蛇葡萄素、白藜芦醇的相对丰度进行分析发现,施用烯酰吗啉后,其含量均受到影响。通过KEGG通路富集分析发现差异代谢物质主要富集在类黄酮生物合成通路、黄酮和黄酮醇的生物合成通路、烟酸盐和烟酰胺代谢通路、类固醇生物合成通路4条代谢通路中。【结论】施用烯酰吗啉后,葡萄叶片代谢受到直接和间接影响。农业生产中合理按照推荐浓度施用烯酰吗啉能对葡萄产生一定的正面效应,从而促进其品质及质量的提升。【Objective】To explore the effects of dimethomoph,a carboxylic amidesfungicide,on the differences of metabolites and metabolic pathways on grape leaves,so as to provide reference for further clarifying the effects of carboxy-lic amides on metabolism of grape leaves.【Method】Healthy Red Globe grape which had not been applied by fungicides underrain shelter culture was taken as the research object.During the growth and development period,it was sprayed with dimethomoph pesticide(DI)and the same amount of sterile water was as control(CK).Ultra performance liquid chroma-tography-tandem mass spectrometry(UPLC-MS)technique was used to research metabolic differences in healthy grape leaves under two different treatments.【Result】The results of partial least squares regression discriminant analysis and displacement test showed that there were significant differences in metabolites between the two groups of grape samples(P<0.05,the same below).Further,orthogonal partial least squares discriminant analysis combined with displacement test found that there were different metabolites in the two groups of grape leaves,and 39 main metabolites causing signifi-cant differences in the metabolism of grape leaves between the two groups were screened.The differential metabolites sig-nificantly changed after treatment with dimethomoph were mainly divided into seven categories:lipids and lipid mole-cules,phenylpropane and polyketones,organic acids and their derivatives,organic oxygen compounds,alkaloids and their derivatives,organic nitrogen compounds and other compounds.The contents of key antagonistic hormones salicylic acid and jasmonic acid,resistance related substances ampelopsin and epsilon-viniferin were analyzed.The results showed that the above substances contents were affected by the application of dimethomoph.Through the enrichment analysis of KEGG pathway,it was found that the differential metabolites were mainly enriched in four metabolic pathways:flavonoid biosynthesis pathway,flavonoid and flavonol biosynthesis

关 键 词：葡萄 烯酰吗啉 差异代谢物 代谢通路 

分 类 号：S436.631.1[农业科学—农业昆虫与害虫防治]