超声辅助提取葡萄籽中原花青素工艺及抗氧化活性研究  

Study on Ultrasound-assisted Extraction of Procyanidins fromGrape Seed Husks and Antioxidant Activity

作　　者：梅瀚 曹金凤 刘世巍[1] 马建龙[2] 丁建海[1] Mei Han;Cao Jinfeng;Liu Shiwei;Ma Jianlong;Ding Jianhai(College of Chemistry and Chemical Engineering,Ningxia Normal University,Guyuan 756000;College of Chemistry and Chemical Engineering,Ningxia University,Yinchuan 750021,China)

机构地区：[1]宁夏师范学院化学化工学院,宁夏固原756000 [2]宁夏大学化学化工学院,宁夏银川750021

出　　处：《广东化工》2023年第5期38-41,共4页Guangdong Chemical Industry

基　　金：宁夏重点研发计划项目(2019BFH02005);宁夏师范学院2020年一流本科课程建设培育项目(分析化学)(NXSFYLKC202010)。

摘　　要：本文在单因素试验的基础上,通过设计正交实验优化葡萄籽中原花青素超声提取工艺条件,并研究了原花青素的抗氧化活性。结果表明:最佳工艺条件为乙醇浓度70%,液料比1∶25,超声时间20 min,提取2次,在此条件下原花青素的提取率为4.96%。所得原花青素提取物对ABTS、DPPH、羟基自由基都具有一定的清除率,IC_(50)值分别是0.390、0.242、0.256μg/mL。Based on the single factor experiment,this paper optimized the ultrasonic extraction process conditions of proanthocyanidins from grape seeds by designing orthogonal experiments,and studied the antioxidant activity of proanthocyanidins.The results showed that the best process conditions were ethanol concentration of 70%,liquid-to-material ratio of 1∶25,ultrasonic time of 20 min,and two extractions.Under these conditions,the extraction rate of proanthocyanidins was 4.96%.The obtained proanthocyanidin extract has a certain scavenging rate for ABTS,DPPH,and hydroxyl radicals,and the IC_(50) values are 0.390,0.242,and 0.256μg/mL,respectively.

关 键 词：葡萄籽 原花青素 正交实验 超声波辅助提取 抗氧化 

分 类 号：TQ[化学工程]