海氏肠球菌与糖蜜联合接种改善葡萄枝叶发酵品质及哈萨克羊养殖效果评价  

Improvement of Fermentation Quality of Grape Branch and Leaf by Combined Inoculation of Enterococcus hirae and Molasses and Evaluation of Breeding Effect of Kazakh Sheep

作　　者：陈永成 杨泽毅 李肖 王旭哲[1] 张凡凡[1] 马春晖[1] CHEN Yongcheng;YANG Zeyi;LI Xiao;WANG Xuzhe;ZHANG Fanfan;MA Chunhui(College of Animal Science and Technology,Shihezi University,Shihezi 833000,China;Sichuan New Hope Animal Nutrition Technology Co.,Ltd.,Chengdu 610000,China)

机构地区：[1]石河子大学动物科技学院,石河子833000 [2]四川新希望动物营养科技有限公司,成都610000

出　　处：《动物营养学报》2023年第3期1771-1780,共10页CHINESE JOURNAL OF ANIMAL NUTRITION

基　　金：财政部和农业农村部-国家现代农业产业技术体系项目(CARS);新疆维吾尔自治区优质饲草产业技术体系项目(2022XJSC-Z-02)。

摘　　要：本试验旨在探究提高葡萄枝叶青贮发酵品质的菌剂添加方法,并以此调制裹包葡萄枝叶青贮,明确其替换部分玉米青贮和玉米秸秆对哈萨克羊生长性能、屠宰性能、血清生化指标及经济效益的影响,为丰富秸秆资源提供基础。发酵试验设4个处理,CK处理(不添加任何菌剂)、EH处理(添加5×10^(6)CFU/g FM海氏肠球菌)、M处理(添加3%FM糖蜜)、EHM处理(联合添加5×10^(6)CFU/g FM海氏肠球菌和3%FM糖蜜),室温发酵60 d后开袋检测营养成分、发酵品质和主要微生物数量。饲喂试验选取20只3月龄体重[(30.0±0.7)kg]相近的哈萨克羊,随机分为2组,每组10只羊。2组分别饲喂玉米青贮、玉米秸秆型全混合日粮(CY组)和葡萄枝叶青贮型全混合日粮(SP组)。预试期7 d,正试期60 d。结果表明:1)与葡萄枝叶原料相比,EHM处理中性洗涤纤维(NDF)、酸性洗涤纤维(ADF)含量和pH及霉菌数量显著降低(P<0.05),乳酸含量与乳酸菌数量显著增加(P<0.05),EHM处理青贮效果最优。2)CY组与SP组之间生长性能、屠宰性能和血清生化指标差异均不显著(P>0.05)。3)与CY组相比,SP组饲料成本显著降低(P<0.05),育肥利润显著升高(P<0.05)。由此可见,海氏肠球菌与糖蜜联合添加可有效改善葡萄枝叶青贮发酵品质,抑制大肠杆菌和霉菌生长,促进乳酸菌繁殖与乳酸含量;且育肥哈萨克羊饲粮中以葡萄枝叶青贮替换部分玉米青贮与玉米秸秆可降低饲料成本,提高经济效益。The aim of this experiment was to explore the bacteria agent adding methods for improve the grape branch and leaf silage fermentation quality,and then prepare the wrapped package grape branch and leaf silage,clear the grape branch and leaf silage replacing part of corn silage and corn straw on the growth performance,slaughter performance,serum biochemical indexes and economic benefits of Kazakh sheep,provide the basis for the rich straw resources.Four treatments were used in the fermentation experiment,CK treatment(without adding any bacterial agent),EH treatment(supplemented with 5×10^(6) CFU/g FM Enterococcus hirae),M treatment(supplemented with 3%FM molasses),EHM treatment(combined supplemented with 5×10^(6) CFU/g FM Enterococcus hirae and 3%FM molasses),after 60 days of fermentation at room temperature,the bag was opened to detect the nutritional composition,fermentation quality and main microbial number.In the feeding experiment,twenty 3-month-old Kazakh sheep with similar body weight[(30.0±0.7)kg]were randomly divided into 2 groups with 10 sheep per group.Sheep in 2 groups were fed corn silage and corn straw type total mixed ration(CY group)and grape branch and leaf silage type total mixed ration(SP group),respectively.The pre-experimental period lasted for 7 days,and the experimental period lasted for 60 days.The results showed as follows:1)compared with the grape branch and leaf raw material,the contents of neutral detergent fiber(NDF),acid detergent fiber(ADF)and pH and mold number of EHM treatment were significantly decreased(P<0.05),the lactic acid content and lactic acid bacteria number were significantly increased(P<0.05),and the silage effect of EHM treatment was optimal.2)There were no significant differences on growth performance,slaughter performance and serum biochemical indexes between CY group and SP group(P>0.05).3)Compared with CY group,the feed cost of SP group was significantly decreased(P<0.05),and the fattening profit was significantly increased(P<0.05).In conclusion,the combined addi

关 键 词：葡萄枝叶 海氏肠球菌 发酵品质 哈萨克羊 养殖效果 

分 类 号：S826[农业科学—畜牧学]