丁酸钠对哺乳期湖羊羔羊生长性能、血浆指标和瘤胃发育的影响  

Effects of Sodium Butyrate on Growth Performance,Plasma Indexes,Rumen Development of Hu Lambs during Suckling Period

作　　者：纪雯雯 云燕 张艳芳 张衍震 李浙烽 王翀 茅慧玲 JI Wenwen;YUN Yan;ZHANG Yanfang;ZHANG Yanzhen;LI Zhefeng;WANG Chong;MAO Huiling(College of Animal Science and Technology,College of Veterinary Medicine,Zhejiang A&F University,Hangzhou 311300,China;Hangzhou Kangdequan Technology Co.,Ltd.,Hangzhou 311107,China)

机构地区：[1]浙江农林大学动物科技学院·动物医学院,杭州311300 [2]杭州康德权科技有限公司,杭州311107

出　　处：《动物营养学报》2023年第3期1803-1815,共13页CHINESE JOURNAL OF ANIMAL NUTRITION

基　　金：国家自然科学基金项目(31902179,32172742)。

摘　　要：本试验旨在研究丁酸钠对哺乳期湖羊羔羊生长性能、血浆指标和瘤胃发育的影响。选用40只初生湖羊羔羊,于7日龄时按体重相近原则分为2组,每组5个重复,每个重复4只羔羊。丁酸钠组(SB组)口腔灌服1.8 mL/kg BW丁酸钠,对照组(Con组)灌服相同体积的生理盐水。试验期35 d。结果表明:1)与Con组相比,SB组的29~35日龄干物质采食量显著升高(P<0.05),7~35日龄平均日增重显著升高(P<0.05)。2)与Con组相比,SB组的血浆生长激素含量显著升高(P<0.05),D-乳酸含量显著降低(P<0.05);SB组的血浆超氧化物歧化酶和过氧化氢酶活性显著升高(P<0.05),丙二醛含量则显著降低(P<0.05);SB组的血浆免疫球蛋白A和免疫球蛋白G含量显著升高(P<0.05),肿瘤坏死因子-α含量显著降低(P<0.05)。3)与Con组相比,SB组的瘤胃总挥发性脂肪酸(TVFA)、乙酸和丁酸含量显著升高(P<0.05)。4)与Con组相比,SB组的瘤胃上皮中胰岛素生长因子-Ⅰ(IGF-Ⅰ)、胰岛素生长因子-Ⅰ受体(IGF-ⅠR)、胰岛素生长因子结合蛋白-5(IGFBP-5)、闭锁小带蛋白-1(ZO-1)、封闭蛋白-1(Claudin-1)、单羧酸转运蛋白1(MCT1)、钠氢离子交换蛋白3(NHE3)、3-羟基-3-甲基戊二酰辅酶A(HMGCL)的mRNA相对表达量显著升高(P<0.05),B淋巴细胞瘤-2相关X蛋白(Bax)的mRNA相对表达量显著降低(P<0.05)。5)瘤胃TVFA含量与瘤胃上皮中Claudin-1、HMGCL的mRNA相对表达量呈显著或极显著正相关(P<0.05或P<0.01),与Bax的mRNA相对表达量呈极显著负相关(P<0.01)。瘤胃丁酸含量与瘤胃上皮中IGF-Ⅰ、IGF-ⅠR、IGFBP-5和钠氢离子交换蛋白1(NHE1)的mRNA相对表达量呈显著或极显著正相关(P<0.05或P<0.01),与Bax的mRNA相对表达量呈负相关(P<0.05)。由此可见,添加丁酸钠可以改善羔羊抗氧化能力和免疫功能,抑制瘤胃上皮细胞凋亡相关基因的表达,促进瘤胃上皮细胞增殖发育、挥发性脂肪酸吸收和代谢相关基因的表达,改善羔羊的瘤胃发酵This study was conducted to investigate the effects of sodium butyrate on growth performance,plasma indexes,rumen development of Hu lambs during suckling period.Forty newborn Hu lambs were selected and randomly divided into 2 groups according to the principle of similar body weight,there were 5 replicates in each group and 4 lambs in each replicate.Lambs in the sodium butyrate group(SB group)were orally administered 1.8 mL/kg BW sodium butyrate,and other in the control group(Con group)were orally administered the same volume normal saline.The experiment lasted for 35 days.The results showed as follows:1)compared with the Con group,the dry matter intake during 29 to 35 days of age of SB group was significantly increased(P<0.05),and the average daily gain during 7 to 35 days of age was significantly increased(P<0.05).2)Compared with the Con group,the plasma growth hormone content of SB group was significantly increased(P<0.05),and the D-lactate content was significantly decreased(P<0.05);the activities of total superoxide dismutase and catalase in plasma of SB group were significantly increased(P<0.05),and the malondialdehyde content was significantly decreased(P<0.05);the contents of immunoglobulin A and immunoglobulin G in plasma of SB group were significantly increased(P<0.05),and the tumor necrosis factor-αcontent was significantly decreased(P<0.05).3)Compared with the Con group,the contents total volatile fatty acid(TVFA),acetic acid and butyric acid in rumen of SB group were significantly increased(P<0.05).4)Compared with the Con group,the mRNA relative expression levels of insulin-like growth factorⅠ(IGF-Ⅰ),insulin-like growth factorⅠreceptor(IGF-ⅠR),insulin-like growth factor binding protein-5(IGFBP-5),zonula occludens-1(ZO-1),Claudin-1,monocarboxylate transporter isoform 1(MCT1),sodium/proton exchanger isoform 3(NHE3)and 3-hydroxy-3-methylglutaryl-CoA lyase(HMGCL)in rumen epithelium of SB group were significantly increased(P<0.05),and the B-cell lymphoma-2-associated X protein(Bax)mRNA relative ex

关 键 词：丁酸钠 生长性能 血浆指标 基因 瘤胃发酵 

分 类 号：S826[农业科学—畜牧学]