复合酸化剂对伊拉兔空肠消化酶活性、免疫功能和紧密连接蛋白表达的影响  

Effects of Compound Acidifier on Digestive Enzyme Activity,Immune Function and Tight Junction Protein Expression in Jejunum of Ira Rabbits

作　　者：杨国峰 贺佳仪 狄斌 谢秋萍[2] 钟辛悦 王长康[1] 高玉云[1] YANG Guofeng;HE Jiayi;DI Bin;XIE Qiuping;ZHONG Xinyue;WANG Changkang;GAO Yuyun(College of Animal Science(College of Bee Science),Fujian Agriculture and Forestry University,Fuzhou 350002,China;Fujian Provincial Green Food Development Center,Fuzhou 350002,China)

机构地区：[1]福建农林大学动物科学学院(蜂学学院),福州350002 [2]福建省绿色食品发展中心,福州350002

出　　处：《动物营养学报》2023年第3期1948-1956,共9页CHINESE JOURNAL OF ANIMAL NUTRITION

基　　金：国家自然科学基金项目(31802079);福建省家禽产业体系岗位专家(KKE19013A,2019-2022)。

摘　　要：本试验旨在研究复合酸化剂对伊拉兔空肠消化酶活性、免疫功能和紧密连接蛋白表达的影响。选取192只体重相近、健康的(35±2)日龄断奶商品代伊拉兔公兔,随机分为4组,每组6个重复,每个重复8只。4组分别为空白组(基础饲粮)、抗生素组(基础饲粮+60 mg/kg盐霉素)、试验Ⅰ组(基础饲粮+0.1%复合酸化剂)和试验Ⅱ组(基础饲粮+0.2%复合酸化剂),复合酸化剂的有效成分为甲酸和丙酸。试验期28 d。结果表明:1)与空白组相比,试验Ⅰ组空肠黏膜胰蛋白酶活性显著提高(P<0.05);各组间空肠黏膜脂肪酶和α-淀粉酶活性无显著差异(P>0.05)。2)与空白组相比,抗生素组、试验Ⅰ组和试验Ⅱ组空肠黏膜白细胞介素-6(IL-6)和白细胞介素-1β(IL-1β)含量显著降低(P<0.05),空肠黏膜白细胞介素-10(IL-10)含量有提高趋势(P=0.086);各组间空肠黏膜分泌型免疫球蛋白A(sIgA)含量无显著差异(P>0.05)。3)与空白组相比,抗生素组、试验Ⅰ组和试验Ⅱ组空肠黏膜IL-1βmRNA相对表达量显著降低(P<0.05);各组间空肠黏膜IL-6和IL-10 mRNA相对表达量无显著差异(P>0.05)。4)与空白组相比,抗生素组和试验Ⅰ组空肠黏膜闭合小环蛋白-1(ZO-1)和闭锁蛋白(occludin)的mRNA相对表达量显著提高(P<0.05),抗生素组和试验Ⅱ组空肠黏膜闭合蛋白-1(claudin-1)的mRNA相对表达量显著降低(P<0.05);各组间相对表达量闭合蛋白-3(claudin-3)的mRNA相对表达量无显著差异(P>0.05)。综上所述,饲粮添加0.1%复合酸化剂可提高伊拉兔空肠黏膜胰蛋白酶活性,增强免疫能力,促进紧密连接蛋白的表达,其效果与抗生素相似,具有良好的替抗潜力。This experiment was conducted to investigate the effects of compound acidifier on digestive enzyme activity,immune function and tight junction protein expression in jejunum of Ila rabbits.A total of 192 healthy(35±2)-day-old weaned male commercial Ira rabbits with similar body weight were randomly divided into 4 groups with 6 replicates per group and 8 rabbits per replicate.The four groups were the blank group(basal diet),the antibiotic group(basal diet+60 mg/kg salinomycin),experimental groupⅠ(basal diet+0.1%compound acidifier)and experimental groupⅡ(basal diet+0.2%compound acidifier).The effective components in compound acidifier were formic acid and propionic acid.The experiment lasted for 28 days.The results showed as follows:1)compared with the blank group,the trypsin activity in jejunum mucosa in experimental groupⅠwas significantly increased(P<0.05),and there were no significant differences in the activities of lipase andα-amylase in jejunal mucosa among all groups(P>0.05).2)Compared with the blank group,the contents of interleukin-6(IL-6)and interleukin-1β(IL-1β)in jejunal mucosa in the antibiotic group,experimental groupⅠand experimental groupⅡwere significantly decreased(P<0.05),and the interleukin-10(IL-10)content in jejunal mucosa had a tendency to be increased(P=0.086).There were no significant differences in the secreted immunoglobulin A(sIgA)content in jejunal mucosa among all groups(P>0.05).3)Compared with the blank group,the IL-1βmRNA relative expression level in jejunal mucosa in the antibiotic group,experimental groupⅠand experimental groupⅡwas significantly decreased(P<0.05),and there were no significant differences in the mRNA relative expression levels of IL-6 and IL-10 in jejunal mucosa among all groups(P>0.05).4)Compared with the blank group,the mRNA relative expression levels of zona occludens-1(ZO-1)and occludin in jejunal mucosa in the antibiotic group and experimental groupⅠwere significantly increased(P<0.05),and the claudin-1 mRNA relative expression level in jej

关 键 词：伊拉兔 复合酸化剂 空肠 消化酶 免疫 紧密连接蛋白 

分 类 号：S816.7[农业科学—饲料科学]