白术多糖缓解环磷酰胺诱导的岭南黄鸡氧化应激和肝脏细胞凋亡  

Polysaccharide of Atractylodes macrocephala Koidz Alleviates Cyclophosphamide Induced Oxidative Stress and Apoptosis in Liver of Lingnan Yellow Chickens

作　　者：陈浩祥 杨舒展 陆智儿 钱隆 李冰心 李婉雁 田允波 黄运茂 许丹宁 曹楠 CHEN Haoxiang;YANG Shuzhan;LU Zhier;QIAN Long;LI Bingxin;LI Wanyan;TIAN Yunbo;HUANG Yunmao;XU Danning;CAO Nan(College of Animal Science and Technology,Zhongkai University of Agriculture and Engineering,Guangzhou 510225,China;Guangdong Province Key Laboratory of Waterfowl Healthy Breeding,Guangzhou 510225,China;Technical Center,Guangzhou Customs,Guangzhou 510000,China)

机构地区：[1]仲恺农业工程学院动物科技学院,广州510225 [2]广东省水禽健康养殖重点实验室,广州510225 [3]广州海关技术中心,广州510000

出　　处：《动物营养学报》2023年第3期1976-1984,共9页CHINESE JOURNAL OF ANIMAL NUTRITION

基　　金：广东省自然科学基金面上项目(20220501605);湖南省自然科学基金面上项目(2021JJ30312)。

摘　　要：本研究旨在探索白术多糖(PAMK)对环磷酰胺(CTX)诱导的岭南黄鸡氧化应激和肝脏细胞凋亡异常的调节作用。选用240只健康1日龄岭南黄鸡,随机分成4个组,每组6个重复,每个重复10只鸡。对照组和环磷酰胺组(CTX组)饲喂基础饲粮,白术多糖组(PAMK组)和白术多糖+环磷酰胺组(PAMK+CTX组)饲喂添加400 mg/kg PAMK的基础饲粮。在20、21、22日龄时,CTX组和PAMK+CTX组的岭南黄鸡腿肌注射20 mg/kg CTX,对照组和PAMK组的岭南黄鸡腿肌注射0.5 mL生理盐水。试验期27 d。结果显示:与CTX组相比,PAMK+CTX组肝脏细胞凋亡现象有所缓解,肝脏细胞状态有所恢复,炎性浸润程度有所降低,点状坏死灶数量明显减少;此外,血清丙二醛(MDA)含量显著下降(P<0.05),血清超氧化物歧化酶(SOD)、谷胱甘肽过氧化物酶(GSH-Px)活性显著升高(P<0.05),肝脏组织半胱氨酸蛋白酶3(Caspase3)的蛋白相对表达量和半胱氨酸蛋白酶9(Caspase9)的mRNA相对表达量显著下调(P<0.05)。由此可见,PAMK能够缓解由CTX引起的岭南黄鸡氧化应激和肝脏细胞凋亡增多的现象。The purpose of this study was to explore the regulatory effects of polysaccharide of Atractylodes macrocephala Koidz(PAMK)on cyclophosphamide(CTX)induced liver oxidative stress and abnormal apoptosis in liver of Lingnan yellow chickens.Two hundred and forty healthy 1-day-old Lingnan yellow chickens were randomly divided into 4 groups with 6 replicates per group and 10 chickens per replicate.Chickens in control group and polysaccharide of Atractylodes macrocephala Koidz group(PAMK group)were fed a basal diet,and the others in cyclophosphamide group(CTX group)and polysaccharide of Atractylodes macrocephala Koidz+cyclophosphamide group(PAMK+CTX group)were fed a basal diet supplemented with 400 mg/kg PAMK.At 20,21 and 22 days of age,the Lingnan yellow chickens in CTX group and PAMK+CTX group were injected with 20 mg/kg CTX into leg muscles,and the Lingnan yellow chickens in control group and PAMK group were injected with 0.5 mL normal saline into leg muscles.The experiment lasted for 27 days.The results showed that compared with CTX group,the apoptosis of liver in PAMK+CTX group was alleviated and the state of liver cells was restored,the degree of inflammatory infiltration was reduced,and the number of punctate necrotic foci was obviously reduced;in addition,the serum malondialdehyde(MDA)content was significantly decreased(P<0.05),the activities of superoxide dismutase(SOD)and glutathione peroxidase(GSH-Px)in serum were significantly increased(P<0.05),and the protein relative expression level of cysteinyl aspartate specific proteinase 3(Caspase3)and mRNA relative expression level of cysteinyl aspartate specific proteinase 9(Caspase9)in liver tissue were significantly down-regulated(P<0.05).In conclusion,the PAMK can alleviate the oxidative stress and increase of liver cell apoptosis caused by CTX in Lingnan yellow chickens.

关 键 词：岭南黄鸡 白术多糖 环磷酰胺 肝脏 氧化应激 细胞凋亡 

分 类 号：S831[农业科学—畜牧学]