干旱胁迫下大苞萱草WRKY基因家族成员鉴定及生物信息学分析  

Identification and Bioinformatics Analysis of WRKY Gene Family Members in Hemerocallis middendorffii under Drought Stress

作　　者：陈丽飞[1] 李嘉峻 刘云怡慧 白云 孟缘 李江 周蕴薇 CHEN Lifei;LI Jiajun;LIU Yunyihui;BAI Yun;MENG Yuan;LI Jiang;ZHOU Yunwei(College of Forestry and Grassland Science,Jilin Agricultural University,Changchun 130118,China;Dingxi Institute of Soil and Water Conservation,Dingxi 743000,China;College of Horticulture,Jilin Agricultural University,Changchun 130118,China)

机构地区：[1]吉林农业大学林学与草学学院,吉林长春130118 [2]定西市水土保持科学研究所,甘肃定西743000 [3]吉林农业大学园艺学院,吉林长春130118

出　　处：《河南农业科学》2023年第2期113-123,共11页Journal of Henan Agricultural Sciences

基　　金：吉林省长春市科技局项目(21ZGN08);吉林省科技厅自然科学基金项目(20200201029JC);吉林省科技厅重点研发项目(20210202081NC);吉林农业大学科研启动基金(202023298)。

摘　　要：WRKY是植物中最大的转录因子家族之一,参与植物的生长发育以及响应生物和非生物胁迫,在植物对干旱胁迫反应的调控网络中发挥着重要作用。对干旱胁迫下大苞萱草在根和叶中的转录组进行测序并分析数据,鉴定大苞萱草WRKY(HmWRKY)基因家族成员并分析其理化性质、蛋白质二级结构、保守基序以及组织表达特性。结果表明,共鉴定出HmWRKY基因家族成员41个,其中包括Ⅰ类、Ⅱ类和Ⅲ类,Ⅱ类可分为Ⅱa、Ⅱb、Ⅱc、Ⅱd、Ⅱe亚类。HmWRKY基因家族成员的氨基酸数量为92~641,理论等电点为4.97~9.99,总平均亲水性均为负值,因此HmWRKY蛋白均为亲水性蛋白。通过对HmWRKY基因表达量分析发现,干旱胁迫下叶中共有10个上调基因,29个下调基因,根中共有20个上调基因,21个下调基因。根据差异基因的表达分析,HmWRKY9基因可能参与大苞萱草的抗旱性调控,确定为候选基因,为进一步研究其功能提供参考。WRKY is one of the largest transcription factor families in plants,which can participate in the growth and development of plants as well as the response to biotic and abiotic stresses,and also plays an important role in the regulatory network of plants in response to drought stress.The transcriptomes of Hemerocallis middendorffii in roots and leaves under drought stress were sequenced and the data were analyzed.The WRKY gene family members were identified and their physicochemical properties,protein secondary structure,conserved motifs and tissue expression analyses were performed in Hemerocallis middendorffii.The results showed that a total of 41 members of WRKY gene family of Hemerocallis middendorffii were identified,including classⅠ,classⅡand classⅢ,and classⅡcould be divided into subclassesⅡa,Ⅱb,Ⅱc,Ⅱd andⅡe.The number of amino acids of WRKY gene family members in Hemerocallis middendorffii was 92—641 and the theoretical isoelectric point was 4.97—9.99.The total average hydrophilicity was negative,so HmWRKY proteins were all hydrophilic proteins.Through the analysis of HmWRKY gene expression,it was found that there were 10 up‐regulated genes and 29 down‐regulated genes in leaves,20 up‐regulated genes and 21 down‐regulated genes in roots.According to the expression of differential genes,HmWRKY9 may be involved in the regulation of drought resistance of Hemerocallis middendorffii,and is identified as a candidate gene,which provides reference for further study of its functions.

关 键 词：大苞萱草 WRKY基因家族 生物信息学 干旱胁迫 

分 类 号：S682.1[农业科学—观赏园艺]