荧光流式细胞仪的标准化研究进展  

Progress in Standardization of Fluorescence Flow Cytometer

作　　者：吕英楷 刘文丽[1] 胡志雄[1] LüYingkai;Liu Wenli;Hu Zhixiong(Center for Medical Metrology,National Institute of Metrology,Beijing 100029,China)

机构地区：[1]中国计量科学研究院医学计量中心,北京100029

出　　处：《激光与光电子学进展》2023年第4期28-40,共13页Laser & Optoelectronics Progress

基　　金：国家重点研发计划(2021YFC2401400)。

摘　　要：目前,关于荧光流式细胞仪的计量和溯源方面的资料相对有限。为了研究基于荧光检测的流式细胞仪的技术发展并加快其标准化进程,对国内外文献资料进行整合,并总结了荧光流式细胞仪的类别、应用、相关标准化研究进展和关键参数的详细信息,主要包括仪器分辨率、散射光和荧光灵敏度、荧光线性相关系数、检出限、准确性、可重复性和稳定性等。国内外对广泛使用的几种评价荧光流式细胞仪性能的计量方法具有基本一致的标准。各个研究组织与应用领域对于流式细胞仪的性能具有不同的需求,一套完整可溯源的表征流式细胞术的计量标准及其评价方法能够方便实验室之间进行可重复和可比较的研究交流与讨论。Currently,resources to improve the metrology and traceability of fluorescence flow cytometer are relatively limited.To study the technical development of flow cytometer based on fluorescence detection and accelerate progress toward standardization,this paper integrates domestic and foreign literatures.Furthermore,detailed information regarding types of fluorescence flow cytometers,their applications,standardization research progresses,and critical parameters including instrument resolution,scattered light and fluorescence sensitivity,fluorescence linear correlation coefficient,detection limit,accuracy,repeatability,and stability are summarized.Foreign and domestic conventional standards are available for several widely used metrology methods to evaluate fluorescence flow cytometer performance.Various research organizations and application areas have different requirements for flow cytometer performance.A complete and traceable set of metrology standards for characterizing flow cytometry,and corresponding evaluation methods enable reproducible and comparable research communications and discussions between laboratories.

关 键 词：荧光检测 流式细胞仪 标准化 计量 溯源 

分 类 号：TH773[机械工程—仪器科学与技术]