Auxin induces lateral root formation in Bupleurum: A heme oxygenase dependent approach  

作　　者：Liang Feng Hua Chen Jun Zhao Dan Liu Zhen Wei Yuchan Li Bin Yang Yilian He Meng Zhang Dabin Hou Ma Yu 

机构地区：[1]School of Life Science and Engineering,Southwest University of Science and Technology,Mianyang 621010,China [2]Laboratory of Medicinal Plant Cultivation,Institute of Medicinal Plant Development,Chinese Academy of Medical Sciences&Peking Union Medical College,Beijing 100193,China [3]Institute of Biomass Energy,Neijiang Academy of Agricultural Sciences of Sichuan Province,Neijiang 641000,China

出　　处：《Chinese Herbal Medicines》2023年第1期57-62,共6页中草药（英文版）

基　　金：financially supported by China Agriculture Research System of MOF and MARA,the CAMS Innovation Fund for Medical Sciences(CIFMS)(No.2016-I2M-2-003);Opening Project Fund of Key Laboratory of Rubber Biology and Genetic Resource Utilization,Ministry of Agriculture/State Key Laboratory Breeding Base of Cultivation&Physiology for Tropical Crops/Danzhou Investigation&Experiment Station of Tropical Crops,Ministry of Agriculture(No.RRL-KLOF202201)。

摘　　要：Objective: The content of saikosaponins in genus Bupleurum is increased with numbers of lateral root, but the genetic mechanisms are largely unknown. This study aims to identify the heme oxygenase(HO) gene family members of B. chinense and B. scorzonerifolium, and assess their role in the root development in Bupleurum.Methods: The gene sequences of HO family were selected from iso-seq full-length transcriptome data of B. chinense and B. scorzonerifolium, and were analyzed in physicochemical properties, conserved domains,motifs and phylogenetic relationship. In addition, the expression patterns of HO gene in different parts of roots were compared via transcriptome sequencing and qRT-PCR in the two species.Results: Five Bupleurum HO genes(BcHO1-BcHO5) belonging to the HO1 subfamily were identified from the transcriptome data, whereas the HO_(2) subfamily member was not identified. The expression levels of BcHO1 and BcHO_(2) were significantly higher than those of other three HO members in the transcriptome analysis. In addition, the expression profile of BcHO1 showed consistency with lateral root development in B. chinense and B. scorzonerifolium.Conclusion: Hos might participate in the auxin-induced morphogenesis of lateral roots. The yield of saikosaponin may be improved by manipulating expression of these genes.

关 键 词：AUXIN Bupleurum L. heme oxygenase lateral root formation 

分 类 号：R283[医药卫生—中药学]