Engineering Multipedal DNA Walker on Paper for Sensitive Electrochemical Detection of Plant MicroRNA  

作　　者：Xiaojuan Liu Junzhu Xiang Hao Cheng Yuying Wang Feng Li 

机构地区：[1]College of Chemistry and Pharmaceutical Sciences,Qingdao Agricultural University,Qingdao,Shandong 266109,China [2]College of Plant Health&Medicine,Qingdao Agricultural University,Qingdao,Shandong 266109,China

出　　处：《Chinese Journal of Chemistry》2022年第23期2808-2814,共7页中国化学（英文版）

基　　金：financially supported by the National Natural Science Foundation of China(No.22076090);the Shandong Provincial Natural Science Foundation(ZR2020ZD37);the Shandong Province Higher Educational Program for Young Innovation Talents.

摘　　要：MicroRNAs (miRNAs) are vital regulators in both plants and animals. Therefore, it is highly desirable to develop portable and user-friendly biosensors for convenient and sensitive detection of miRNAs. Herein, a novel paper-based electrochemical biosensor was intelligently engineered for the detection of plant miRNA based on a smart DNA walking machine and λ-exonuclease (λ-Exo)-assisted target recycling amplification. Using TaMIR5086 as a target plant miRNA, the presence of TaMIR5086 could initiate the target recycling process and activate the DNA walker to move along the track on the paper. Then, numerous electroactive molecules-labeled single-strand DNA (ssDNA) would be released and adsorbed onto the surface of screen-printed electrode, generating a remarkably increased electrochemical signal. Benefitting from the dual amplification, the developed biosensor exhibits excellent analytical performance toward TaMIR5086 with a detection limit down to 0.37 pmol/L. Furthermore, the paper-based biosensor could be applied for the analysis of target miRNA in complex biological samples, which found great potential in the fields of miRNA analysis and plant biology research.

关 键 词：RNA recognition ELECTROCHEMISTRY Sensor DNA walker on paper Target recycling amplification 

分 类 号：O646[理学—物理化学]