基于黄酮类成分含量差异的子洲黄芪产地判别模式研究  

Patterns of identifying the production area of Astragali Radix from Zizhou based on the flavonoid content characteristics

作　　者：连天赐 魏胜利[1,2] 冯紫薇 于芳[1,2] 赵婷[1,2] 刘芳[1,2] 田文仓 刘凤波[1] 张媛[1,2] LIAN Tianci;WEI Shengli;FENG Ziwei;YU Fang;ZHAO Ting;LIU Fang;TIAN Wencang;LIU Fengbo;ZHANG Yuan(Beijing University of Chinese medicine,Beijing 102488,China;Standardized production of traditional Chinese Medicine,Engineering Research Center of Ministry of Education,Beijing 102488,China;Lunan pharmaceutical Shandong new era Pharmaceutical Co.,Ltd.,Shandong 273400,China;Beijing Tongrentang health pharmaceutical(Ningxia)Co.,Ltd.,Ningxia 750021,China)

机构地区：[1]北京中医药大学,北京102488 [2]中药材规范化生产教育部工程研究中心 [3]鲁南制药山东新时代药业有限公司 [4]北京同仁堂健康药业宁夏有限公司

出　　处：《北京中医药大学学报》2023年第2期281-288,共8页Journal of Beijing University of Traditional Chinese Medicine

基　　金：北京市科技计划项目(No.Z201100005420005);宁夏回族自治区科技厅重点研发计划项目(No.2020BBF02005)。

摘　　要：目的 通过对不同产地黄芪中黄酮类成分含量的测定和分析,为黄芪优质药材商品子洲黄芪的精准产地鉴别提供实验依据。方法 以陕西子洲及山西浑源、内蒙古自治区、甘肃省的蒙古黄芪为实验材料,采用高效液相色谱-二极管矩阵检测器(HPLC-PDA)测定黄芪中主要黄酮类成分含量;基于0.7~1.0 cm直径段黄芪的黄酮类成分含量数据,通过主成分分析(PCA)、正交偏最小二乘法-判别分析(OPLS-DA)、聚类分析和Fisher判别分析,筛选子洲黄芪与其他产地黄芪的质量差异性标志物,并构建判别模型。结果 获得不同产地黄芪中主要黄酮类成分含量;PCA可基于黄酮类成分含量对子洲黄芪与其他产地黄芪进行初步区分,OPLS-DA确定了4种子洲黄芪与其他产地黄芪的质量差异性标志物,基于质量差异性标志物的不同产地黄芪聚类分析正确率为88.9%,以黄芪紫檀烷苷含量/黄芪异黄烷苷含量和4种黄酮苷含量之和所构建的判别模型对于不同产地黄芪的总体判别率为94.4%。结论 通过黄酮类成分判别不同产地黄芪的方法切实可行。Objective We aimed to determine and analyze the flavonoid contents in Astragali Radix from different habitats, so as to provide an experimental basis for the accurate origin identification of Astragali Radix from Zizhou.Methods The root segments of Astragalus membranaceus(Fisch.) Bge.var. mongholicus(Bge.) Hsiao from Zizhou County of Shaanxi Province, Hunyuan County of Shanxi Province, Inner Mongolia Province, and Gansu Province, were used as experimental materials. High-performance liquid chromatography photo-diode array(HPLC-PDA) was used to determine the main flavonoid contents in Astragali Radix. Based on the flavonoid content data of Astragali Radix from 0.7 to 1.0 cm in diameter, the quality difference markers of Astragali Radix from Zizhou and other habitats were screened and a discrimination model was constructed by principal component analysis(PCA), orthogonal partial least squares discriminant analysis(OPLS-DA), cluster analysis, and Fisher discriminant analysis.Results The main flavonoid contents in Astragali Radix from different habitats were obtained. PCA can be used to preliminarily distinguish between Zizhou Astragali Radix and other habitats based on the flavonoid contents. OPLS-DA revealed four quality difference markers of Astragali Radix from Zizhou and other habitats. The accuracy of cluster analysis of Astragali Radix from different habitats based on the quality difference markers was 88.9%. The total discrimination rate of Astragali Radix from different habitats was 94.4% by the discrimination model based on the ratio of astrapterocarpan glucoside to isomucronulatol glucoside and the sum of the contents of four flavonoid glycosides.Conclusion The method to identify Astragali Radix from different production areas based on flavonoid contents is feasible.

关 键 词：子洲黄芪 产地 黄酮类成分 

分 类 号：R282[医药卫生—中药学]