低强度电刺激对神经损伤性ED大鼠勃起功能影响  

Effect of low-intensity electrical stimulation on erectile function in ED rats with nerve injury

作　　者：李鸿斌[1] 冯东 侯琳 邢建东[2] 张延兵 LI Hongbin;FENG Dong;HOU Lin;XING Jiandong;ZHANG Yanbing(Department of Urology,the Fifth People's Hospital of Datong,Datong,Shanxi 037006,China;Department of Endocrinology,the Fifth People's Hospital of Datong,Datong,Shanxi 037006,China)

机构地区：[1]山西省大同市第五人民医院泌尿外科,山西大同037006 [2]山西省大同市第五人民医院内分泌科,山西大同037006

出　　处：《中国医学工程》2023年第3期14-18,共5页China Medical Engineering

基　　金：山西省重点研发计划项目(201903D321157)。

摘　　要：目的评估低强度电刺激(LIES)对神经损伤性勃起功能障碍(ED)大鼠勃起功能(EF)影响。方法雄性SD大鼠随机分为3组:Sham组、双侧海绵体神经损伤(BCNI)组和LIES+BCNI组,每组10只大鼠。LIES治疗7 d后,通过对比大鼠最大海绵体内压/平均动脉压(ICP_(max)/MAP)评估阴茎EF变化;通过ELISA试剂盒测量海绵体内活性氧(ROS)蛋白含量;通过Western blot方法检查海绵体中转化生长因子-β1(TGF-β1)、白细胞介素-6(IL-6)和C反应蛋白(CRP)蛋白表达;通过免疫荧光染色评估海绵体中α-平滑肌肌动蛋白(α-SMA)蛋白表达。结果与Sham组相比,BCNI组大鼠ICP_(max)/MAP降低(P<0.05);与BCNI组比,BCNI+LIES组大鼠ICP_(max)/MAP升高(P<0.05)。与Sham组相比,BCNI组大鼠海绵体ROS含量增高(P<0.05);与BCNI组比,BCNI+LIES组大鼠海绵体ROS含量降低(P<0.05)。与Sham组相比,BCNI组大鼠海绵体TGF-β1、IL-6和CRP蛋白表达增加(P<0.05);与BCNI组比,BCNI+LIES组大鼠海绵体TGF-β1、IL-6和CRP蛋白表达降低(P<0.05)。与Sham组相比,BCNI组海绵体内α-SMA蛋白表达降低(P<0.05);与BCNI组比,BCNI+LIES组大鼠海绵体α-SMA蛋白表达增加(P<0.05)。结论LIES治疗不仅改善BCNI后大鼠EF,而且对海绵体组织具有保护作用,包括防止氧化应激、炎症和平滑肌细胞丢失。【Objective】To evaluate the effect of low intensity electrical stimulation(LIES)on erectile function(EF)in rats with nerve injury induced erectile dysfunction(ED).【Methods】Male SD rats were randomly divided into 3 groups:Sham group,bilateral cavernous nerve injury(BCNI)group and LIES+BCNI group,with 10 rats in each group.After 7 days of treatment with LIES,the changes of penile EF were evaluated by comparing the maximum intracavernous pressure/mean arterial pressure(ICP_(max)/MAP)of rats.The content of reactive oxygen species(ROS)protein in cavernous body was measured by ELISA kit.The protein expression of transforming growth factor-β1(TGF-β1),interleukin-6(IL-6)and C-reactive protein(CRP)in cavernous body was examined by Western blot.Immunofluorescence staining was used to evaluate theα-smooth muscle actin(α-SMA)protein expression.【Results】Compared with Sham group,ICP_(max)/MAP of rats in BCNI group decreased(P<0.05);compared with BCNI group,ICP_(max)/MAP in BCNI+LIES group increased(P<0.05).Compared with Sham group,the content of ROS in cavernous body of rats in BCNI group increased(P<0.05);compared with BCNI group,the content of ROS in cavernous body of rats in BCNI+LIES group decreased(P<0.05).Compared with Sham group,the expression of TGF-β1,IL-6 and CRP protein in cavernous body of rats in BCNI group increased(all P<0.05);compared with BCNI group,the expression of TGF-β1,IL-6 and CRP protein in cavernous body of rats in BCNI+LIES group decreased(all P<0.05).Compared with Sham group,the expression ofα-SMA protein in BCNI group was decreased(P<0.05);compared with BCNI group,theα-SMA protein expression in cavernous body of BCNI+LIES group was increased(P<0.05).【Conclusion】LIES treatment not only improved EF in rats after BCNI,but also had protective effects on cavernous body,including prevention of oxidative stress,inflammation,and smooth muscle cell loss.

关 键 词：电刺激 勃起功能障碍 海绵体 大鼠 

分 类 号：R698.2[医药卫生—泌尿科学]