麻竹DlmDELLA基因克隆与分析  

Cloning and Analysis of DlmDELLA Gene of Dendrocalamus latiflorus Munro

作　　者：陈刚 王楠楠 白玮元 韩芳英 朱强 Chen Gang;Wang Nannan;Bai Weiyuan;Han Fangying;Zhu Qiang(Basic Forestry and Proteomics Research Center,College of Forestry,Fujian Agriculture and Forestry University,Fuzhou,350002)

机构地区：[1]福建农林大学林学院,基础林学与蛋白质组学研究中心,福州350002

出　　处：《分子植物育种》2023年第6期1874-1883,共10页Molecular Plant Breeding

基　　金：国家自然科学基金面上项目(No.32071847,No.31870660);国家重点研发项目(No.2018YFD0600102);福建省教育厅科技创新团队项目(No.118/KLA18069A);福建省科技厅林木种质资源创新项目(No.118/KLA18069A)共同资助。

摘　　要：DELLA蛋白是GAs(赤霉素)信号转导途径中的关键因子,但在麻竹中相关的研究仍是空白。为了研究麻竹中DlmDELLA基因的功能,实验通过同源克隆的方法,从麻竹DNA和cDNA中克隆出一条DlmDELLA基因,基因全长c DNA序列为1863 bp,无内含子,编码620个氨基酸,分子量为65.57 kD,等电点为5.20。蛋白聚类分析,发现麻竹DlmDELLA编码的蛋白与其他植物DELLA蛋白一样具有完整的功能结构域,而且麻竹DELLA蛋白与玉米、水稻、毛竹等禾本科植物的亲缘关系较近。利用STRING数据库对其互作蛋白进行分析发现其与GIDs、PIF3、SLY1等蛋白可能有相互作用。RT-qRCR分析麻竹DlmDELLA的时空表达,发现其在叶、胚芽组织中表达量较高;在外源GA3处理下,基因表达呈现上升趋势,在24 h表达量达到最高;外源PAC处理下,基因表达在3 h上升到最高后下降。原生质体中基因的亚细胞定位,发现DlmDELLA蛋白定位在细胞核中。本研究为进一步研究麻竹中关于GAs信号通路中DELLA蛋白功能及其分子调控机制提供一定的理论依据和参考价值。DELLA protein is a key factor in the GAs(gibberellin)signal transduction pathway.But related research in Ma Bamboo is still blank.In order to study the function of the DlmDELLA gene in Ma Bamboo,we cloned a DlmDELLA gene from Ma Bamboo’s DNA and cDNA through the method of homologous cloning.The full-length cDNA sequence of the gene is 1863 bp,without introns,encoding 620 amino acids,the molecular weight is 65.57 kD,and the isoelectric point is 5.20.Protein cluster analysis revealed that the protein encoded by DlmDELLA has complete functional domains like other plant DELLA proteins,and DlmDELLA protein is closely related to Monocot such as maize,rice,and moso bamboo.Using STRING database to analyze its interacting proteins and found that it may interact with GIDs,PIF3,SLY1 and other proteins.The qRT-RCR analysis of the temporal and spatial expression of DlmDELLA in Ma Bamboo,found that its expression level was higher in leaf and embryo tissues.Under the exogenous GA3 treatment,the gene expression showed an upward trend,and the expression level reached the highest at 24 h.Under the exogenous PAC treatment,the gene expression decreased after rising to the highest at 3 h.The subcellular localization of genes in protoplasts revealed that DlmDELLA was localized in the nucleus.This study provides a certain theoretical basis and reference value for further research on the function of DELLA protein in GAs signaling pathway and its molecular regulation mechanism in Ma Bamboo.

关 键 词：麻竹(Dendrocalamus latiflorus Munro) DELLA蛋白 基因时空表达模式 亚细胞定位 

分 类 号：S795[农业科学—林木遗传育种]