96份花椰菜霜霉病苗期抗性接种鉴定与分子标记检测  

Inoculation Identification and Molecular Marker Detection of 96 Cauliflower DownyMildew Resistance at Seedling Stage

作　　者：黄雷 刘春晴 姚雪琴[1] 李光庆[1] 吴鑫燕 谢祝捷[1] Huang Lei;Liu Chunqing;Yao Xueqin;Li Guangqing;Wu Xinyan;Xie Zhujie(Horticultural Research Institute,Shanghai Academy of Agricultural Sciences,Shanghai,201403)

机构地区：[1]上海市农业科学院设施园艺研究所,上海201403

出　　处：《分子植物育种》2023年第6期1929-1936,共8页Molecular Plant Breeding

基　　金：上海市科技兴农重点攻关项目(沪农科攻字[2016]第6-1-5号);国家重点研发计划项目(2017YFD0101805)共同资助。

摘　　要：为获得最适花椰菜霜霉病苗期鉴定方法,创制花椰菜霜霉病抗性种质资源,以4个不同浓度和3个接种量的霜霉病原菌悬浮液对4个花椰菜品种进行苗期人工接种,结果表明当接种浓度为3.5×10^(5)个孢子/m L,接种量为100μL时可以准确反映花椰菜各个品种的抗感病类型。同时,利用该接种方法对96份花椰菜材料进行苗期抗病性鉴定,筛选得到19份抗性材料。结合Ppa3、Pp523和Ppa^(207)等抗病基因位点紧密连锁的分子标记检测其分布情况,其中含有基因Pp523、Ppa^(207)、Ppa3的材料分别有11、9和1份,含有其他抗性基因位点的材料有3份,含有两个或两个以上抗性基因的材料仅有6份。综上分析,说明携带霜霉病抗性基因的材料较少且不同材料之间的抗病位点差异较大,同时利用该方法可以在早期快速筛选含有目标抗性基因的材料,提高抗病育种效率。In order to obtain the best identification method for downy mildew of cauliflower,creation of cauliflower downy mildew resistant germplasm,four cauliflower varieties were inoculated artificially at seedling stage with 4 downy mildew pathogen suspensions of different concentrations and 3 inoculations.The results showed that when the inoculation concentration was 3.5×10^(5)spores/mL,at 100μL,the types of disease resistance in cauliflower varieties can be accurately reflected.Meanwhile,the inoculation method was used to identify the disease resistance of 96 cauliflower materials in seedling stage,19 resistant materials were screened.The distribution of resis tant gene loci,such as Ppa3,Pp523 and Ppa^(207),contains 11,9 and 1 copies of genetic Pp523,Ppa^(207)and Ppa3,three copies of materials containing other resistance gene sites,only 6 materials containing two or more resistance genes.Based on the above analysis,it shows that the materials carrying downy mildew resistance genes are less and the re sistance sites of different materials are different.At the same time,this method can be used to quickly screen materials containing target resistance genes in the early stage,improve the efficiency of disease resistance breeding.

关 键 词：花椰菜 霜霉病 接种鉴定 分子标记 

分 类 号：S436.35[农业科学—农业昆虫与害虫防治]