坐骨神经来源外泌体促进大鼠周围神经损伤后轴突再生及功能恢复  

Sciatic nerve derived exosomes in promotion of axon regeneration and functional recovery following peripheral nerve injury in rat

作　　者：黄金生 张葛毅 李江南 李森瑞 路来金[2] 周南 HUANG Jinsheng;ZHANG Geyi;LI Jiangnan;LI Senrui;LU Laijin;ZHOU Nan(Department of Orthopaedics,The First Affiliated Hospital of Zhengzhou University,Zhenzhou 450000,China;Department of Hand and Foot Surgery,The First Hospital of Jilin University,Changchun 130031,China)

机构地区：[1]郑州大学第一附属医院骨科,郑州450000 [2]吉林大学第一医院手足外科,长春130031

出　　处：《中华显微外科杂志》2023年第1期95-100,共6页Chinese Journal of Microsurgery

基　　金：国家自然科学基金(82071388);中国博士后科学基金(2019M660175);河南省优秀青年科学基金(212300410077)。

摘　　要：目的研究坐骨神经来源外泌体(SN-EXO)促进周围神经损伤(PNI)后轴突再生加速功能恢复的作用及机制。方法 2021年3月至2022年10月,郑州大学第一附属医院骨科通过EdU细胞增殖实验评价SN-EXO对许旺细胞(SCs)增殖能力的影响。21只健康雄性SD大鼠随机分为假手术(Sham)组、PNI组及SN-EXO治疗组,每组7只。Sham组仅显露右侧坐骨神经,PNI组及SN-EXO治疗组大鼠右侧坐骨神经挤压后神经外膜下分别注射PBS及SN-EXO。术后28 d进行步态分析和坐骨神经功能指数(SFI)检测,并处死各组大鼠取右侧坐骨神经,通过HE染色评价坐骨神经组织病理学变化及轴突排列情况,采用免疫荧光染色分析SCs及轴突再生情况。所得数据进行统计学分析,P<0.05为差异有统计学意义。结果 SN-EXO可显著增强SCs增殖能力,差异有统计学意义(P<0.05)。术后28 d,SN-EXO治疗组SFI为(-27.65±4.36)分,高于PNI组(-57.33±7.49)分,差异有统计学意义(P<0.05);SN-EXO治疗组轴突排列较PNI组更加致密且有序,损伤所致轴突崩解和空泡变性现象较少;SN-EXO治疗组NF200及S100β荧光强度高于PNI组,差异均有统计学意义(P<0.05)。结论 SN-EXO通过增强SCs增殖能力促进PNI后轴突再生及功能恢复。Objective To explore the effect of sciatic nerve derived exosomes(SN-EXO)on axon regeneration and functional recovery after peripheral nerve injury(PNI).Methods From March 2021 to October 2022,the Department of Orthopedics of the First Affiliated Hospital of Zhengzhou University studied the effect of SN-EXO on the proliferation of Schwann cells(SCs)through EdU cell proliferation experiment.Twenty-one healthy male SD rats were randomly divided into 3 groups of sham operation,peripheral nerve injury(PNI)and SN-EXO treatment,with 7 rats in each group.The right sciatic nerves of rat models in sham group were exposed without injury.In the rat in PNI group and SN-EXO treatment group,PBS and SN-EXO were injected under the epineurium of right sciatic nerves following sciatic nerve crush.Sciatic nerve function index(SFI)was performed at 28 days after operation,and then sacrificed.Right sciatic nerves were removed for further exploration of nerve regeneration.The histopathological changes and axon arrangement of sciatic nerves were evaluated by HE staining.Regeneration efficiency of neurofilaments and SCs were obserred by NF200 and S100βdouble staining of sciatic nerve.The data obtained were statistically analyzed,and P<0.05 was statistically significant.Results It was found that SN-EXO can significantly enhance the proliferation ability of SCs,with statistically significant difference(P<0.05).SFI in SN-EXO treatment group and PNI group were(-27.65±4.36)and(-57.33±7.49),respectively,and the difference was statistically significant(P<0.05).Axons in SN-EXO treatment group were arranged more closely and orderly than those in the PNI group at 28 days after operation,and there were less injury induced axon disintegration and vacuolation.Immunofluorescence assay indicated that NF200 and S100βfluorescence intensity in SN-EXO treatment group was significantly higher than that in the PNI group,and the difference was statistically significant(P<0.05).Conclusion SN-EXO could enhance the proliferation of SCs to promote axon regene

关 键 词：坐骨神经来源外泌体 许旺细胞 周围神经再生 神经功能恢复 

分 类 号：R688[医药卫生—骨科学]