河南省濮阳市某小学一起聚集性上呼吸道感染疫情病原学分析  

Pathogenic characteristics of a clustering upper respiratory tract infection in a primary school cases in Puyang city,Henan province

作　　者：李广康 张清杰[1] 张玉华[1] 徐国锋[1] 王爱梅[1] 常艳林 赵俊君[1] LI Guangkang;ZHANG Qingjie;ZHANG Yuhua;XU Guofeng;WANG Aimei;CHANG Yanlin;ZHAO Junjun(Puyang Center for Disease Control and Prevention,Puyang,Henan 457000,China)

机构地区：[1]濮阳市疾病预防控制中心,河南濮阳457000

出　　处：《河南预防医学杂志》2023年第2期130-132,共3页Henan Journal of Preventive Medicine

摘　　要：目的 分析河南省濮阳市某小学一起聚集性呼吸道感染病例的病原学特征。方法 选取该小学83名学生为研究对象,分为发病组、密接组、对照组三组,采集研究对象口咽拭子标本采用多重PCR法进行病原学核酸检测,并对结果进行分析。结果 27种呼吸道病原体核酸检测结果中乙型流感病毒、肺炎链球菌和流感嗜血杆菌呈现阳性。乙型流感病毒核酸检测发病组阳性率(94.12%)明显高于密接组(11.11%)和对照组(0.00%)(P<0.001),发病组、密接组和对照组肺炎链球菌阳性率分别为70.59%、55.56%和56.67%(P>0.05),流感嗜血杆菌阳性率分别为88.24%、72.22%和73.33%(P>0.05)。其他病原体未检出。多重PCR法及MDCK细胞和鸡胚病毒培养均表明乙型流感病毒为Victoria系。结论 该小学上呼吸道感染病例的病原体主要是乙型流感病毒Victoria系,是由乙型流感病毒引起的暴发疫情。Objective To analyze the pathogenic characteristics of a clustering upper respiratory tract infection in a primary school in Puyang city, Henan. Methods A total of 83 students in the primary school were selected as the research objects, and divided into three groups to analyze: the case group, the close contact group, and the control group. Samples of oropharyngeal swabs were collected for pathogenic nucleic acid detection by multiplexing PCR, and the results were analyzed. Results Of the nucleic acid test results of 27 respiratory pathogens, influenza B virus, streptococcus pneumoniae and haemophilus influenzae were positive. The positive rate of influenza B virus nucleic acid detection in the case group(94.12%) was significantly higher than that in the close contact group(11.11%) and the control group(0.00%)(P<0.001). In the case group, close contact group,and control group, the positive rates of streptococcus pneumoniae were 70.59%, 55.56% and 56.67%(P>0.05), respectively, and the positive rates of haemophilus influenzae were 88.24%, 72.22% and 73.33%(P>0.05). There were no other pathogens detected.Multiplexing PCR and MDCK cells and chicken embryo virus culture showed that influenza B virus was Victoria strain.Conclusion Influenza B virus Victoria strain was the main pathogen of upper respiratory tract infection in the primary school,and the cluster as an outbreak caused by influenza B virus.

关 键 词：上呼吸道感染 口咽拭子 多重荧光定量PCR 病原学特征 

分 类 号：R725.1[医药卫生—儿科] R181.3[医药卫生—临床医学]