Properties of Collagen/Sodium Alginate Hydrogels for Bioprinting of Skin Models  

作　　者：Tian Jiao Qin Lian Weilong Lian Yonghui Wang Dichen Li Rui L.Reis Joaquim Miguel Oliveira 

机构地区：[1]State Key Laboratory for Manufacturing System Engineering,Xi’an Jiaotong University,Xi’an,710049,China [2]Shaanxi Ketao-AM Technology Co.,Ltd.,Xi’an,710100,China [3]B’s Research Group,I3Bs—Research Institute on Biomaterials,Biodegradables and Biomimetics,Headquarters of the European Institute of Excellence on Tissue Engineering and Regenerative Medicine,University of Minho,Barco,4805-017,Guimarães,Portugal [4]ICVS/3B’s–PT Government Associate Laboratory,Barco,4805-017,Guimarães,Portugal

出　　处：《Journal of Bionic Engineering》2023年第1期105-118,共14页仿生工程学报（英文版）

基　　金：funded by the National Key R&D Program of China(2018YFE0207900);People's Liberation Army(BWS17J036,18-163-13-ZT-003-011-01);the National Natural Science Foundation of China(51835010 and 51375371);Xi’an Science and Technology Plan Project(21ZCZZHXJS-QCY6-0012);Shaanxi Science and Technology Project(2022KXJ-147)。

摘　　要：3D printing technology has great potential for the reconstruction of human skin.However,the reconstructed skin has some differences from natural skin,largely because the hydrogel used does not have the appropriate biological and physical properties to allow healing and regeneration.This study examines the swelling,degradability,microstructure and biological properties of Collagen/Sodium Alginate(Col/SA)hydrogels of differing compositions for the purposes of skin printing.Increasing the content of sodium alginate causes the hydrogel to exhibit stronger mechanical and swelling properties,a faster degradation ratio,smaller pore size,and less favorable biological properties.An optimal 1%collagen hydrogel was used to print bi-layer skin in which fibroblasts and keratinocytes showed improved spreading and proliferation as compared to other developed formulations.The Col/SA hydrogels presented suitable tunability and properties to be used as a bioink for bioprinting of skin aiming at finding applications as 3D models for wound healing research.

关 键 词：Bioink Bioprinting-Collagen sodium alginate hydrogel-Skin Wound healing BIONIC 

分 类 号：Q81[生物学—生物工程]