LncRNA NEAT1通过靶向miR-103a-3p/SDF2轴调节滋养层细胞增殖、凋亡和侵袭  

LncRNA NEAT1 regulates the proliferation,apoptosis and invasion of trophoblast cells by targeting miR-103a-3p/SDF2 axis

作　　者：纪龙花 韩毓 陈莲芳 陈婵 韦先梅 许文丽 王莉[3] Ji Longhua;Han Yu;Chen Lianfang(Department of Obstetrics and Gynecology,Haikou Fourth People's Hospital,Haikou 571100)

机构地区：[1]海口市第四人民医院妇产科,海口571100 [2]海口市人民医院(中南大学湘雅医学院附属海口医院)妇产科,海口570208 [3]海南省人民医院妇产科,海口570311

出　　处：《现代妇产科进展》2023年第3期171-176,共6页Progress in Obstetrics and Gynecology

基　　金：海南省卫生健康委员会科研课题(No:21A200131)。

摘　　要：目的:探讨lncRNA NEAT1在子痫前期(PE)患者胎盘组织中的表达及对滋养层细胞增殖、凋亡和侵袭的影响及作用机制。方法:qRT-PCR检测lncRNA NEAT1在PE患者胎盘组织中的表达,将人绒毛滋养层细胞HTR-8/SVneo随机分为对照(control)组、pcDNA-NC组、pcDNA-NEAT1组、si-NC组、si-NEAT1组、si-NEAT1+inhibitor NC组、si-NEAT1+miR-103a-3p inhibitor组,分别检测各组HTR-8/SVneo细胞增殖、凋亡、侵袭和迁移情况,Western blot检测SDF2蛋白表达。双荧光素酶报告基因验证LncRNA NEAT1、SDF2与miR-103a-3p的靶向关系。结果:LncRNA NEAT1在PE患者胎盘组织中表达水平升高。与pcDNA-NC组比较,pcDNA-NEAT1组lncRNA NEAT1表达、SDF2蛋白表达、细胞凋亡率显著升高,miR-103a-3p表达及细胞增殖活性、迁移和侵袭能力显著降低(P<0.05)。与si-NC组比较,si-NEAT1组lncRNA NEAT1表达、SDF2蛋白表达、细胞凋亡率显著降低,miR-103a-3p表达及细胞增殖活性、迁移和侵袭能力显著升高(P<0.05)。下调miR-103a-3p表达可明显减弱沉默lncRNA NEAT1表达对HTR-8/SVneo细胞增殖、迁移与侵袭的促进作用,并增加细胞凋亡(P<0.05)。双荧光素酶检测结果显示,lncRNA NEAT1、SDF2与miR-103a-3p有靶向关系。结论:LncRNA NEAT1在PE胎盘组织中高表达,沉默lncRNA NEAT1表达可通过上调miR-103a-3p,抑制SDF2表达,促进滋养层细胞增殖与侵袭。Objective:To investigate the expression of lncRNA NEAT1 in placental tissue of patients with preeclampsia(PE)and its influences on the proliferation,apoptosis and invasion of trophoblast cells and its mechanism.Methods:qRT-PCR was used to detect the expression of lncRNA NEAT1 in placental tissue of patients with preeclampsia.Human villous trophoblast cells HTR-8/SVneo were randomly divided into control group,pcDNA-NC group,pcDNA-NEAT1 group,si-NC group,si-NEAT1 group,si-NEAT1+inhibitor NC group,and si-NEAT1+miR-103a-3p inhibitor group.The proliferation,apoptosis,invasion and migration of HTR-8/SVneo cells in each group were detected,respectively.Western blot was used to detect the SDF2 protein expression.Dual-luciferase reporter gene was used to verify the targeting relationship between lncRNA NEAT1,SDF2 and miR-103a-3p.Results:The expression level of lncRNA NEAT1 was increased in the placental tissue of PE patients.Compared with the pcDNA-NC group,the expression of lncRNA NEAT1,the expression of SDF2 protein and cell apoptosis rate in the pcDNA-NEAT1 group were significantly increased,the expression of miR-103a-3p and cell proliferation activity,migration and invasion abilities were significantly decreased(P<0.05).Compared with the si-NC group,the expression of lncRNA NEAT1,the expression of SDF2 protein,and the apoptosis rate of the si-NEAT1 group were significantly decreased,the expression of miR-103a-3p and cell proliferation activity,migration and invasion abilities were significantly increased(P<0.05).Down-regulation of miR-103a-3p expression significantly attenuated the promotion of silencing lncRNA NEAT1 expression on the proliferation,migration and invasion of HTR-8/SVneo cells,and increased cell apoptosis(P<0.05).The results of dual luciferase assay showed that lncRNA NEAT1 and SDF2 had a targeting relationship with miR-103a-3p.Conclusion:LncRNA NEAT1 is highly expressed in placental tissue of preeclampsia,and silencing the expression of lncRNA NEAT1 can up-regulate miR-103a-3p,inhibit the expression of

关 键 词：LncRNA NEAT1 miR-103a-3p 子痫前期 凋亡 侵袭 

分 类 号：R816.91[医药卫生—放射医学]