防控温郁金细菌性枯萎病的生物有机肥研制与应用  

Development and application of bio-organic fertilizers for prevention and control of bacterial wilt of Curcuma wenyujin

作　　者：冯林 周铨 陈卫良[1] 毛碧增[1] FENG Lin;ZHOU Quan;CHEN Weiliang;MAO Bizeng(Key Laboratory of Biology of Crop Pathogens and Insects of Zhejiang Province,Ministry of Agriculture and Rural Affairs Key Lab of Molecular Biology of Crop Pathogens and Insects,Institute of Biotechnology,Zhejiang University,Hangzhou 310058,China)

机构地区：[1]浙江大学生物技术研究所,农业农村部作物病虫分子生物学重点实验室,浙江省作物病虫生物学重点实验室,浙江杭州310058

出　　处：《浙江农业学报》2023年第3期630-638,共9页Acta Agriculturae Zhejiangensis

基　　金：农业农村部农业重大技术协同推广项目(2020XTTGZYC02);浙江省“三农六方”科技协作计划(CTZB-F160728AWZ-SNY1-37)。

摘　　要：茄科劳尔氏菌(Ralstonia solanaceraum)引起的温郁金(Curcuma wenyujin Y.H.Chen et C.Ling)细菌性枯萎病是目前温郁金种植中的毁灭性病害。本文利用2株高效拮抗菌Paenibacillus jamila ZJU-C612-3和Bacillus amyloliquefaciens ZJU-C612-4研制生物有机肥,并进行田间防治效果试验。温室盆栽试验结果显示,施生物有机肥的温郁金发病率下降了60百分点;田间试验显示,每667 m 2施200 kg生物有机肥作为基肥的田块中,健康根茎的发病率降低17.66百分点,且植株生长健壮。此外,施生物有机肥能缓解温郁金的连作障碍,在5 a连作田块施该肥后,在病害高发期的7月和8月,连作根茎的发病率分别下降9.43百分点和19.75百分点。研究结果可为今后防治温郁金细菌性枯萎病的专用生物有机肥的产业化开发提供依据。Bacterial wilt of Curcuma wenyujin(BWCW)caused by Ralstonia solanaceraum was becoming a common destructive disease in the planting of C.wenyujin at present.In order to control the disease,a bio-organic fertilizer(BOF)was developed by using Paenibacillus jamila ZJU-C612-3 and Bacillus amyloliquefaciens ZJU-C612-4,which were screened early in our laboratory and showed strong antagonistic activities to R.solanaceraum.The incidence rate of C.wenyujin tuber treated with BOF in greenhouse pot culture was reduced by 60 percentage point.After using 200 kg BOF in 667 m 2 field,the incidence rate of healthy C.wenyujin tuber was reduced by 17.66 percentage point,and the plants grew well.In addition,the BOF could alleviate the monocropping obstacle of C.wenyujin.After applying bio-organic fertilizer in the 5-year continuous cropping field,the incidence rate of continuous cropping C.wenyujin tuber decreased by 9.43 percentage point and 19.75 percentage point in July and August.This study result could provide a basis for the industrialized development of bio-organic fertilizer for the control of bacterial wilt of C.wenyujin in future.

关 键 词：温郁金 茄科劳尔氏菌 生物有机肥 细菌性枯萎病 发病率 

分 类 号：S476[农业科学—农业昆虫与害虫防治]