基于UPLC-MS/MS代谢组学技术分析归芪益母口服液对产后奶牛血浆代谢物的影响  

Effect of Guiqi Yimu Oral Liquid on Plasma Metabolites in Postpartum Dairy Cows Based on UPLC-MS/MS Metabolomics Technology

作　　者：孔维怡 王菲菲 岳康 秦俊杰[2] 朱浩[2] 郭延生[1] KONG Weiyi;WANG Feifei;YUE Kang;QIN Junjie;ZHU Hao;GUO Yansheng(College of Agriculture,Ningxia University,Yinchuan 750021,China;Beijing Center Biology Co.,Ltd.,Beijing 102600,China)

机构地区：[1]宁夏大学农学院,银川750021 [2]北京生泰尔科技股份有限公司,北京102600

出　　处：《中国畜牧兽医》2023年第3期913-923,共11页China Animal Husbandry & Veterinary Medicine

基　　金：国家自然科学基金(31860719)。

摘　　要：【目的】通过超高效液相色谱-质谱法(UPLC-MS/MS)代谢组学技术分析归芪益母口服液对产后奶牛血浆代谢物的影响,以阐明归芪益母口服液对产后奶牛的保健机制。【方法】选取15头体况相近、2~3胎健康待产荷斯坦奶牛,随机分为对照组(n=7)和试验组(n=8),分别在产后立即灌服饮用水和归芪益母口服液,500 mL/头,连续灌服6 d。产后7 d晨饲前采血,经预处理后采用UPLC-MS/MS上机检测,利用MetaboAnanlyst 5.0软件筛选VIP≥1、P<0.05的差异代谢物进行代谢通路富集分析。【结果】产后7 d,试验组血浆代谢轮廓较对照组发生明显改变,筛选出16种差异代谢物,其中8种显著上调,包括蛋氨酸亚砜、磷酸肌酸、甘油磷酸胆碱、皮质醇、L-色氨酸、溶血磷脂酰胆碱(18∶2)、五羟色胺和L-精氨酸;8种显著下调,包括1-氨基环己酸、3-甲基巴豆酰甘氨酸、L-天冬酰胺、单酰甘油酯酰基(16∶1)、泛酰巯基乙胺、十八碳单烯酰胺、1-甲基鸟嘌呤和3-N-甲基-L-组氨酸。差异代谢物显著富集在甘油磷脂代谢、精氨酸和脯氨酸代谢、色氨酸代谢通路。【结论】归芪益母口服液对产后奶牛具有保健作用,可能与其调节甘油磷脂代谢、皮质醇代谢、色氨酸代谢、精氨酸和脯氨酸代谢从而发挥抗炎、抗氧化、调节食欲及肠道菌群稳态、缓解能量负平衡作用有关。【Objective】The purpose of this experiment was to analyze the effects of Guiqi Yimu oral liquid on plasma metabolites in postpartum dairy cows by UPLC-MS/MS metabolomics technology,in order to clarify the health-care mechanism of Guiqi Yimu oral liquid on postpartum dairy cows.【Method】A total of 15 healthy Holstein dairy cows with similar body condition and 2-3 parity were selected and randomly divided into control group(n=7)and experiment group(n=8).500 mL/head Guiqi Yimu oral liquid and drinking water were administered continuously for 6 days.Blood was collected before morning feeding on 7 d postpartum.After pretreatment,UPLC-MS/MS was used for on-machine detection,and MetaboAnanlyst 5.0 software was used to screen differential metabolites with VIP≥1 and P<0.05 for metabolic pathway enrichment analysis.【Result】At 7 days postpartum,the plasma metabolism profile in experiment group was significantly changed compared with control group.Sixteen differential metabolites were screened out,of which eight were significantly up-regulated,including methionine sulfoxide,creatine phosphate,glycerophosphocholine,cortisol,L-tryptophan,lysophosphatidylcholine(18∶2),5-hydroxytryptamine and L-arginine;Eight were significantly down-regulated,including 1-aminocyclohexanoic acid,3-methylcrotonyl glycine,L-asparagine anhydrous,monoacylglycerolacyl(16∶1),panthenyl mercaptoethylamine,octadecy monoenamide,1-methylguanine and 3-N-methyl-L-histidine.Differential metabolites were significantly enriched in glycerophospholipid metabolism,arginine and proline metabolism,and tryptophan metabolism pathways.【Conclusion】Guiqi Yimu oral liquid had the health-care effect of postpartum dairy cows,which might play the role of anti-inflammatory,antioxidant,appetite-regulating,intestinal bacteria and relieving negative energy balance by regulating glycerophospholipid metabolism,cortisol metabolism,tryptophan metabolism,arginine and proline metabolism.

关 键 词：产后奶牛 归芪益母口服液 代谢组学 血浆代谢物 通路富集 

分 类 号：Q493.2[生物学—生理学]