绒山羊4个多胎性状候选基因多态性及其与产羔数的关联分析  

Polymorphisms of Four Polyembryony Related Genes andIts Association Analysis with Litter Size in Cashmere Goats

作　　者：萨初拉 吴铁成 马跃军 何云梅 朱莉仙 何托雅 武玉平 刘斌 Sachula;WU Tiecheng;MA Yuejun;HE Yunmei;ZHU Lixian;HE Tuoya;WU Yuping;LIU Bin(Inner Mongolia Academy of Agricultural and Husbandry Sciences,Hohhot 010031,China;Etuoke Agricultural and Animal Husbandry Technology Extension Center,Erdos 017000,China;Alashan Animal Husbandry and Veterinary Technology Promotion Center,Alashan 750030,China;Erdos Li Xin Industrial Corporation Limited,Erdos 017000,China;Hangjin Agricultural and Animal Husbandry Technology Extension Center,Erdos 017400,China)

机构地区：[1]内蒙古自治区农牧业科学院,呼和浩特010031 [2]鄂托克旗农牧技术推广中心,鄂尔多斯017000 [3]阿拉善盟畜牧兽医技术推广中心,阿拉善750030 [4]鄂尔多斯市立新实业有限公司,鄂尔多斯017000 [5]杭锦旗农牧技术推广中心,鄂尔多斯017400

出　　处：《中国畜牧兽医》2023年第3期1037-1047,共11页China Animal Husbandry & Veterinary Medicine

基　　金：内蒙古创新基金项目(2020CXJJM01);内蒙古自治区科技计划项目(2020GG0095);内蒙古自治区科技成果转化引导项目(2020CG0100);鄂尔多斯市绒山羊重大专项(2019);内蒙古自治区绒山羊重大专项(2017)。

摘　　要：【目的】通过对内蒙古白绒山羊4个多胎性状相关基因进行测序和生物信息学分析,深入挖掘与产羔数显著相关的单核苷酸多态位点(single nucleotide polymorphisms,SNP)位点,为提升绒山羊高效繁育提供理论依据。【方法】选取阿拉善型、阿尔巴斯型绒山羊母羊244只,利用MultipSeq多重PCR结合二代高通量技术检测生长分化因子9(growth differentiation factor 9,GDF9)、骨形态发生蛋白15(bone morphogenetic protein 15,BMP15)、骨形态发生蛋白受体1B(bone morphogenetic protein receptor 1B,BMPR1B)和β-1,4-N-乙酰氨基半乳糖转移酶2(beta-1,4-N-acetyl-galactosaminyl transferase 2,B4GALNT2)基因多态性,并对不同SNP位点与绒山羊产羔数进行关联分析。【结果】通过GATK分析共预测得到172个SNPs位点,其中BMPR 1 B、B 4 GALNT 2、BMP 15、GDF 9基因相关SNPs位点分别为95、33、26和18个。GLM模型关联分析发现,10个SNPs位点与白绒山羊产羔数显著相关(P<0.05),进一步进行卡方检验发现,其中7个SNPs位点基因型与产羔数显著相关(P<0.05)。BMPR 1 B基因g.29893723 C>G、g.29897064 G>A、g.29897722 G>A、g.29897734 C>A和g.29938673 C>G位点的CC、GG、GA、CA和CC基因型个体产羔数分别显著高于CG、GA、GG、CC和GG基因型(P<0.05);B 4 GALNT 2基因g.37072289 G>A位点的GG和GA基因型个体产羔数均显著高于AA基因型(P<0.05);GDF 9基因g.66027842 A>C位点的CC和AC基因型个体产羔数均显著高于AA基因型(P<0.05)。【结论】试验获得了内蒙古白绒山羊多胎性状相关基因GDF 9、BMP 15、BMPR 1 B和B 4 GALNT 2的SNPs位点序列特征,发现了与白绒山羊产羔数显著相关的10个SNPs位点,并揭示了不同基因型产羔数间的差异,从而为内蒙古白绒山羊分子辅助育种提供了有力的SNP参考位点。【Objective】Through sequencing and bioinformatics analysis of 4 polyembryony related genes in Inner Mongolia White cashmere goats,the single nucleotide polymorphisms(SNP)significantly related to litter size were deeply explored,so as to provide a theoretical basis for improving cashmere goat breeding efficiency.【Method】244 cashmere goat ewes in type Alashan and Aerbasi were selected as candidates,and MultipSeq PCR combined with second-generation high-throughput sequencing technology was used to detect the polymorphism of growth differentiation factor 9(GDF9),bone morphogenetic protein 15(BMP15),bone morphogenetic protein receptor 1B(BMPR1B)and beta-1,4-N-acetyl-galactosaminyl transferase 2(B4GALNT2)genes,the association between different SNPs and litter size of cashmere goats were analyzed.【Result】A total of 172 SNPs were predicted by GATK analysis,including 95,33,26 and 18 SNPs related to BMPR 1 B,B 4 GALNT 2,BMP 15 and GDF 9 genes,respectively.The correlation analysis results of GLM model showed that 10 SNPs were significantly correlated with the litter size(P<0.05),and among them genotypes of 7 SNPs illustrated significantly related with litter size with further chi-square test(P<0.05).The litter size of CC,GG,GA,CA and CC genotypes of BMPR1B gene at g.29893723 C>G,g.29897064 G>A,g.29897722 G>A,g.29897734 C>A and g.29938673 C>G were significantly higher than that of CG,GA,GG,CC and GG genotypes(P<0.05),respectively.The litter size of GG and GA genotypes of B 4 GALNT 2 gene at g.37072289 G>A were significantly higher than that of AA genotype(P<0.05),and the litter size of CC and AC genotypes of GDF 9 gene at g.66027842 A>C were significantly higher than that of AA genotype(P<0.05).【Conclusion】The sequence characteristics of SNPs of GDF 9,BMP 5,BMPR 1 B and B 4 GALNT 2 genes related to multiparity traits in Inner Mongolia White cashmere goats were obtained through the study,and a total of 10 SNPs were significantly related to litter size in cashmere goats,and revealed the difference among litter s

关 键 词：绒山羊 GDF 9基因 BMP 15基因 BMPR 1 B基因 B 4 GALNT 2基因 产羔数 

分 类 号：S813.3[农业科学—畜牧学]