一株蛋鸡血清8b型禽腺病毒的分离鉴定  

Isolation and Identification of a Serotype 8b Avian Adenovirus from Laying Chicken

作　　者：路晓 武景琦 张帆 韩易航 牟建青 梁贤 王芸 曹兵 于可响[1] 宋敏训[1] LU Xiao;WU Jingqi;ZHANG Fan;HAN Yihang;MU Jianqing;LIANG Xian;WANG Yun;CAO Bing;YU Kexiang;SONG Minxun(Institute of Poultry,Shandong Academy of Agricultural Sciences,Jinan 250100,China;College of Veterinary Medicine,Inner Mongolia Agricultural University,Hohhot 010018,China;Shandong Agricultural University,Taian 271018,China;Yantai Aishijin Animal Health Products Co.,Ltd.,Yantai 264003,China;Shandong Delino Bioengineering Co.,Ltd.,Zibo 256400,China;Liaocheng Academy of Agricultural Sciences,Liaocheng 252000,China;Tengzhou Animal Husbandry andFisheries Development Centre,Tengzhou 275000,China)

机构地区：[1]山东省农业科学院家禽研究所,山东济南250100 [2]内蒙古农业大学兽医学院,内蒙古呼和浩特010018 [3]山东农业大学,山东泰安271018 [4]烟台爱士津动物保健品有限公司,山东烟台264003 [5]山东德利诺生物工程有限公司,山东淄博256400 [6]聊城市农业科学院,山东聊城252000 [7]滕州市畜牧渔业事业发展中心,山东滕州277500

出　　处：《家禽科学》2023年第3期1-5,I0006,共6页Poultry Science

基　　金：烟台市科技发展计划(2021NYNC021);山东省科技型中小企业创新能力提升工程项目(2022TSGC2289);山东省现代农业产业技术体系家禽创新团队计划(SDAIT-11-01);山东省农业科学院创新工程项目(CXGC2023A10)。

摘　　要：从山东潍坊地区以包涵体肝炎为病变特征的发病蛋鸡中采集病料,通过接种7日龄SPF鸡胚分离到1株病毒,经PCR检测、测序分析、进化树分析和血清中和试验鉴定,确定为血清8b型禽腺病毒。将该病毒通过肌肉注射接种14日龄SPF鸡,结果仅部分试验鸡出现肝脏轻微坏死变性,这与临床中发病鸡肝脏明显坏死甚至破裂并不相符,分析可能与饲养条件、管理水平、鸡的品种和日龄、药物使用等方面有关。The diseased materials were collected from laying chicken with inclusion body hepatitis as the pathological feature in Weifang,Shandong Province.A virus strain was isolated from 7-day-old SPF chicken embryos,and identified as serum 8b avian adenovirus by PCR detection,sequencing analysis,evolutionary tree analysis and serum neutralization test.The virus was inoculated into 14-day-old SPF chickens by intramuscular injection.The results showed that only some of the experimental chickens had slight liver necrosis and degeneration,which was not consistent with the obvious liver necrosis or even rupture of the diseased chicken in clinical practice.This may be related to the feeding conditions,management level,chicken breeds and age,and drug use.

关 键 词：蛋鸡 血清8b型禽腺病毒 包涵体肝炎 分离鉴定 

分 类 号：S858.312.65[农业科学—临床兽医学]