球囊霉素相关土壤蛋白对钒钛磁铁尾矿生态环境的修复效果研究  

Study on Remediation Effect of Glomalin Related Soil Protein on the Ecological Environment of Vanadium-Titanium Magnetite Tailings

作　　者：卢赛 谷海红 艾艳君[1] 张义璇 李富平 LU Sai;GU Haihong;AI Yanjun;ZHANG Yixuan;LI Fuping(College of Mining Engineering,North China University of Science and Technology,Tangshan 063210,China;Hebei Key Laboratory of Mining Development and Security Technology,Tangshan 063210,China;Hebei Industrial Technology Institute of Mine Ecological Remediation,Tangshan 063210,China;Tangshan Key Laboratory of Resources and Environmental Remote Sensing,Tangshan 063210,China)

机构地区：[1]华北理工大学矿业工程学院,河北唐山063210 [2]河北省矿业开发与安全技术重点实验室,河北唐山063210 [3]河北省矿区生态修复产业技术研究院,河北唐山063210 [4]唐山市资源与环境遥感重点实验室,河北唐山063210

出　　处：《金属矿山》2023年第3期250-258,共9页Metal Mine

基　　金：河北省自然科学基金项目(编号:E2021209152,E2015209300);河北省高等学校青年拔尖人才计划项目(编号:BJ2014029);唐山市科学技术研究与发展计划重点项目(编号:19150247E);唐山市科技研发平台培养计划项目(编号:2020TS003b)。

摘　　要：为探讨丛枝菌根真菌(Arbuscular mycorrhizal fungi,AMF)释放的球囊霉素相关土壤蛋白(Glomalin related soil protein,GRSP)对钒钛磁铁尾矿生态环境的修复作用,开展了紫花苜蓿室内培养实验,通过外源施加不同形态及不同剂量的球囊霉素相关土壤蛋白,研究了其对钒钛磁铁尾矿养分环境、团聚结构、重金属活性及紫花苜蓿生长的影响。结果表明:施加GRSP后钒钛磁铁尾矿养分环境得到明显改善,EEG使钒钛磁铁尾矿中速效磷含量增加了19.7%~60.1%;TG效果则更加显著,碱解氮含量和速效磷含量分别增加了12.2%~58.9%和44.7%~111.3%;此外,外源施加TG后钒钛磁铁尾矿团聚结构的稳定性会显著增加,大团聚体占比、平均重量直径和几何重量直径分别增加了29.36%~114.63%、4.76%~15.87%和9.26%~16.67%,提高了钒钛磁铁尾矿对重金属及养分的固持能力;两种形态GRSP对钒钛磁铁尾矿中不同重金属作用有所差异,EEG能显著降低Cu有效性,DTPA提取态Cu含量降低16.7%,TG主要钝化Cr,DTPA提取态Cr含量减少了72.6%。此外,柠檬酸钠的加入在一定程度上抑制了紫花苜蓿的生长。综合分析表明,球囊霉素相关土壤蛋白可以改变钒钛磁铁尾矿重金属活性、提高团聚体稳定性并改善养分环境,为尾矿矿区生态修复提供了理论依据。In order to investigate the effect of Glomalin related soil protein(GRSP)released by Arbuscular mycorrhizal fungi(AMF)on the ecological restoration of vanadium-titanium magnet tailings,laboratory culture experiments of alfalfa were carried out.Exogenous applied different forms and doses of glomalin related soil protein to study the nutrient environment,aggregate structure and heavy metals availability in vanadium-titanium magnetite tailings.The results showed that the nutrient environment of vanadium-titanium magnetite tailings was significantly improved after GRSP was applied.The content of available phosphorus in vanadium-titanium magnetite tailings increased by 19.7%~60.1%with EEG.TG effect was more significant,and the content of alkali-hydrolyzed nitrogen and available phosphorus increased by 12.2%~58.9%and 44.7%~111.3%,respectively.The increase of phosphoric acid is beneficial to enhance the precipitation of heavy metals.TG improve the stability of the aggregate structure of vanadium-titanium magnetite tailings,it can increase the proportion of large aggregates,the mean weight diameter and geometric mean diameter of vanadium-titanium magnetite tailings by 29.36%~114.63%,4.76%~15.87%and 9.26%~16.67%,respectively,which is beneficial to improve the retention capacity of vanadium-titanium magnetite tailings to heavy metals and nutrients.The effects of two GRSP forms on different heavy metals in vanadium-titanium magnet tailings are different,EEG can significantly reduce the availability of Cu,and the content of Cu extracted from DTPA decreases by 16.7%;TG can passivate Cr,the content of Cr extracted from DTPA decreases by 72.6%.In addition,the addition of sodium citrate inhibited the growth of alfalfa.Comprehensive analysis showed that the GRSP could change the availability of heavy metals and improve nutrient environment and aggregate structure in vanadium-titanium magnetite tailings,which could provide theoretical basis for ecological restoration in tailing areas.

关 键 词：钒钛磁铁尾矿 重金属 球囊霉素相关土壤蛋白 养分 团聚结构 

分 类 号：X703[环境科学与工程—环境工程]