A relatively high zoonotic trematode prevalence in Orientogalba ollula and the developmental characteristics of isolated trematodes by experimental infection in the animal model  

作　　者：Jian Li Yijing Ren Lei Yang Jiani Guo Haiying Chen Jiani Liu Haoqiang Tian Qingan Zhou Weiyi Huang Wei Hu Xinyu Feng 

机构地区：[1]Basic Medical College,Guangxi Traditional Chinese Medical University,Nanning 530005,Guangxi,China [2]College of Life Sciences,Inner Mongolia University,Hohhot 010070,China [3]College of Animal Science and Technology,Guangxi University,Nanning 530005,Guangxi,China [4]National Institute of Parasitic Diseases,Chinese Center for Disease Control and Prevention,Key Laboratory of Parasite and Vector Biology of China Ministry of Health,WHO Collaborating Centre for Tropical Diseases,Joint Research Laboratory of Genetics and Ecology On Parasite-Host Interaction,Chinese Center for Disease Control and Prevention,Fudan University,200025 Shanghai,China [5]Department of Infectious Diseases,Huashan Hospital,State Key Laboratory of Genetic Engineering,Ministry of Education Key Laboratory for Biodiversity Science and Ecological Engineering,Ministry of Education Key Laboratory of Contemporary Anthropology,School of Life Sciences,Fudan University,Shanghai 200438,China [6]School of Global Health,Chinese Center for Tropical Diseases Research,Shanghai Jiao Tong University School of Medicine,Shanghai 20025,China [7]One Health Center,Shanghai Jiao Tong University-The University of Edinburgh,Shanghai 20025,China

出　　处：《Infectious Diseases of Poverty》2022年第4期72-80,共9页贫困所致传染病（英文）

基　　金：Guangxi Traditional Chinese Medical University Scientific Research Project(XP021059);National Parasitic Resources Center(NPRC-2019-194-30);Key Technology Project of Inner Mongolia Science and Technology Department(2021GG0171)。

摘　　要：Background:Food-borne parasitic diseases decrease food safety and threaten public health.The snail species is an intermediate host for numerous human parasitic trematodes.Orientogalba ollula has been reported as intermediate hosts of many zoonotic trematodes.Here,we investigated the prevalence of zoonotic trematodes withinO.ollula in Guangxi,China,and assessed their zoonotic potential.Methods:Snails were collected from 54 sites in 9 cities throughout Guangxi.The snail and trematode larvae species were determined by combining morphological characteristics and molecular markers.The trematodes prevalence and constituent ratio were calculated and compared among different habitat environments.Phylogenetic trees of the trematode species were constructed using the neighbor-joining method with nuclear internal transcribed spacer 2(ITS2)sequences.The developmental cycles of the isolated trematodes were examined by experimental infection in ducks.The developmental characteristics ofEchinostoma revolutum was recorded by dissecting infected ducklings from 1-day post infection(dpi)to 10 dpi.Results:The overall prevalence of trematode larvae was 22.1%(1818/8238)inO.ollula from 11 sample sites.Morphological together with molecular identification,showed thatE.revolutum,Australapatemon sp.,Hypoderaeum conoideum,Pharyngostomum cordatum,andEchinostoma sp.parasitizedO.ollula,with the highest infection rate ofE.revolutum(13.0%).However,noFasciola larvae were detected.The trematodes prevalence and constituent ratio varied in two sub-biotypes(P<0.01).A neighbor-joining tree analysis of ITS2 sequences resulted in distinct monophyletic clades supported by sequences from isolated larvae with high bootstrap values.Ducklings exposed toO.ollula infected withEchinostoma sp.,E.revolutum,andH.conoideum larvae were successfully infected.The animal model forEchinostoma revolutum was successfully established.E.revolutum matured from larvae to adult at 10 dpi in the intestine of the duck,and the developmental characteristics ofE.revolutum were char

关 键 词：Orientogalba ollula Zoonotic trematode Intermediate host PREVALENCE ITS2 

分 类 号：R532.2[医药卫生—内科学] R-332[医药卫生—临床医学]