防风水提物对抗生素加重小鼠过敏性鼻炎的改善作用研究  

Study on the improvement effect of Fangfeng water extract on mice with aggravated allergic rhinitis by antibiotics

作　　者：陈艳春[1] 徐世影[1] 应航宇[2] 陈志凌[1] CHEN Yanchun;XU Shiying;YING Hangyu;CHEN Zhiling(Department of Otorhinolaryngology,Hangzhou Hospital of Traditional Chinese Medicine,Hangzhou 310005,China;不详)

机构地区：[1]杭州市中医院耳鼻咽喉科,310005 [2]杭州市中医院皮肤科,310005

出　　处：《浙江医学》2023年第5期453-459,I0003,共8页Zhejiang Medical Journal

基　　金：浙江省中医药科技计划项目(2021ZA104)。

摘　　要：目的 探讨防风水提物通过免疫调节对抗生素加重小鼠过敏性鼻炎(AR)的改善效果。方法 40只健康雄性Balb/c小鼠随机分为正常组、模型组、抗生素组、防风水提物组、防风水提物+抗生素组,每组8只,除正常组外,使用卵清白蛋白激发小鼠AR。各组灌服相应抗生素混合液和(或)防风水提物14 d。观察小鼠打喷嚏、挠鼻次数用于评价AR症状。计数鼻腔灌洗液中炎症细胞(嗜酸性粒细胞、白细胞、淋巴细胞、中性粒细胞)数量,ELISA法检测血清中致敏因子(免疫球蛋白E、组胺、白三烯)、炎症细胞因子以及鼻腔灌洗液炎症细胞因子水平评价小鼠体内炎症反应;甲苯胺蓝染色计数鼻黏膜组织肥大细胞数量;流式细胞术检测小鼠脾脏CD4+CD25+Foxp3+Treg/CD4+T细胞比例,Western blot法检测小鼠鼻黏膜组织Toll样受体4(TLR4)、TNF受体关联因子6(TRAF6)、NF-κB p65、NF-κB抑制因子(IκBα)、活化IκBα(p-IκBα)蛋白表达情况。结果 与正常组比较,模型组小鼠AR症状被激发,肥大细胞和炎症细胞数量增加,致敏因子水平与体内炎症反应升高,CD4+CD25+Foxp3+Treg/CD4+T细胞比例降低(P<0.05),TLR4、TRAF6、p-IκBα/IκBα、NF-κB p65蛋白表达增加(均P<0.05)。与模型组比较,抗生素组小鼠AR症状加重,肥大细胞浸润和炎症细胞数量增加,致敏因子水平与体内炎症反应升高,CD4+CD25+Foxp3+Treg/CD4+T细胞比例降低(P<0.05),TLR4、TRAF6、p-IκBα/IκBα、NF-κB p65蛋白表达水平均增加(均P<0.05);而防风水提物组小鼠AR小鼠症状减轻,肥大细胞浸润和炎症细胞数量减少,致敏因子水平与体内炎症反应降低,CD4+CD25+Foxp3+Treg/CD4+T细胞比例升高(P<0.05),TLR4、TRAF6、p-IκBα/IκBα、NF-κB p65蛋白表达水平均降低(均P<0.05)。与抗生素组比较,防风水提物+抗生素组小鼠AR症状减轻,致敏因子水平与体内炎症反应降低,CD4+CD25+Foxp3+Treg/CD4+T细胞比例降低(P<0.05),TLR4、TRAF6、pIκBα/IκBαObjective To explore the improvement effect of Fangfeng water extract(Fwe)on allergic rhinitis(AR)symptoms in mice aggravated by antibiotics through immunomodulation.Methods Forty healthy female Balb/c mice were randomly divided into normal group,model group,antibiotic group,Fwe group,Fwe+antibiotic group,eight in each group,except from the normal group,mice were sensitized with ovalbumin(OVA)to motivate AR.Corresponding group mice were gavaged with antibiotic and(or)Fwe for 14 d.Times of sneezing and scratching of mice were used to evaluate AR symptom.The number of inflammatory cells(eosinophils,leukocytes,lymphocytes,neutrophils)in nasal lavage fluid were count,and ELISA was used to detect levels of sensitized factors(immunoglobulin E,histamine,leukotriene C4)and interleukin-2(IL-2),IL-4,IL-5,IL-6,IL-10,IL-17,tumor necrosis factor-α(TNF-α),interferon-γ(IFN-γ),transforming growth factor-β1(TGF-β1)of nasal lavage fluid and serum to evaluate inflammatory in mice.Toluidine blue staining was used to count the number of mast cells in nasal mucosa.Flow cytometry was used to detect CD4+CD25+Foxp3+Treg/CD4+T cells of spleen in mouse.Western blot was used to detect protein expression of toll-like receptor 4(TLR4),TNF receptor associated factor6(TRAF6),nuclear factorκB p65(NF-κB p65),inhibitor of NF-κB(IκBα),pho-IκBα(p-IκBα)in nasal mucosa.Results Compared with normal group,the AR symptoms of mice in the model group were stimulated,number of mast cells and inflammatory cells were increased,content of sensitized factors and inflammatory were increased,CD4+CD25+Foxp3+Treg/CD4+T cells were decreased,protein expression of TLR4,TRAF6,NF-κB p65,IκBα,p-IκBαwere increased(P<0.05).Compared with the model group,the AR symptoms of the mice in the antibiotic group were aggravated,number of mast cells and inflammatory cells were increased,content of sensitized factors and inflammatory were increased,CD4+CD25+Foxp3+Treg/CD4+T cells were decreased,protein expression of TLR4,TRAF6,NF-κB p65,IκBα,p-IκBαwere increa

关 键 词：过敏性鼻炎 防风水提物 抗生素 免疫反应 调节型T细胞 

分 类 号：R285.5[医药卫生—中药学]