HPLC法同时测定延胡索不同部位中11种核苷类成分  

Simultaneous Determination of 11 Nucleosides in Different Parts of Corydalis yanhusuo by HPLC

作　　者：刘焱 黄小兰 曾莹[3] 万哲见 刘世文 祁俊生 周游 周浓 Liu Yan;Huang Xiaolan;Zeng Ying;Wan Zhejian;Liu Shiwen;Qi Junsheng;Zhou You;Zhou Nong(Engineering Laboratory of Chongqing for Green Planting and Deep Processing of Genuine Medicinal Materials in Three Gorges Reservoir Area,College of Biology and Food Engineering,Chongqing Three Gorges University,Chongqing 404000,China;Chengdu Hehe Medical Laboratory Co.,Ltd.,Chengdu 610101,China;Department of Pharmacy,West China Hospital,Sichuan University,Chengdu 610041,China)

机构地区：[1]重庆三峡学院生物与食品工程学院,三峡库区道地药材绿色种植与深加工重庆市工程实验室,重庆404120 [2]成都和合医学检验所有限公司,四川成都610101 [3]四川大学华西医院药剂科,四川成都610041

出　　处：《中国野生植物资源》2023年第3期38-44,共7页Chinese Wild Plant Resources

基　　金：重庆市技术创新与应用示范重点研发项目(cstc2018jscx-mszdX0093);重庆市技术创新与应用示范一般项目(cstc2018jscx-msybX0370)。

摘　　要：目的:测定并评价不同产地延胡索药材不同部位中11种核苷类成分的含量。方法:采用高效液相色谱法测定核苷类成分的含量,并对测定结果进行聚类分析。结果:不同产地延胡索中核苷类物质含量由高到低为鸟苷>尿苷>腺苷>肌苷>胞苷>尿嘧啶>2’-脱氧肌苷>2’-脱氧胸苷>腺嘌呤>胸腺嘧啶>2’-脱氧腺苷,11种核苷总含量整体表现为叶>茎>块茎,不同产地延胡索核苷类总含量由高到低为浙江磐安>浙江东阳>陕西汉中>重庆万州>重庆开州,块茎中的11种核苷成分含量比无显著性差异,但茎和叶差异较大。结论:此方法可用于延胡索不同部位中核苷类成分的测定,为其综合利用提供思路。Objective:To determine and evaluate the contents of 11 nucleosides in different parts of Corydalis yanhusuo from different origins by using HPLC method.Methods:The contents of nucleosides were determined by High Performance Liquid Chromatography(HPLC)and a cluster analysis was carried out for the results.Results:The content order of nucleosides was guanosine uridine adenosine inosine cytidine uracil 2'-deoxyinosine 2'-deoxythymidine adenine thymine 2'-deoxyadenosine in C.yanhusuo from different origins;The order of total nucleosides content in different parts was leaf stem tuber;The order of total nucleosides content of different origins was Pan'an(Zhejiang) Dongyang(Zhejiang) Hanzhong(Shaanxi) Wanzhou(Chongqing) Kaizhou(Chongqing);There was no significant difference in the contents of 11 nucleosides in C.yanhusuo from different areas,but there was a significant difference in stem and leaf.Conclusion:This method can be used for the determination of nucleosides in different parts and it might provide the scientific basis for the comprehensive utilization in C.yanhusuo.

关 键 词：延胡索 核苷 高效液相色谱法 含量测定 同时测定 

分 类 号：Q-9[生物学]