甘肃贝母离体小鳞茎直接发生途径的研究  

Study on the Direct Induction of Bulblets in Vitro Culture of Fritillaria przewalskii Maxim.

作　　者：何玉明 董婉琦 张文楠 高素芳 孙欢 何春雨 张延红 He Yuming;Dong Wanqi;Zhang Wennan;Gao Sufang;Sun Huan;He Chunyu;Zhang Yanhong(College of Pharmacy,Gansu University of Chinese Medicine,Lanzhou 730000,China)

机构地区：[1]甘肃中医药大学药学院,甘肃兰州730000

出　　处：《中国野生植物资源》2023年第3期45-51,共7页Chinese Wild Plant Resources

基　　金：国家自然科学基金(81960683);甘肃省自然科学基金项目(21JR1RA263);兰州市科技计划项目(2019-1-83,2020-ZD-59);甘肃省科技厅民生科技专项-科技特派员专题(20CX9NA070);甘肃省知识产权计划项目(21ZSCQ045);甘肃省高等学校青年博士基金(2021QB-073)。

摘　　要：目的:建立甘肃贝母组织培养小鳞茎的直接发生技术体系。方法:采用单因子试验设计,筛选适宜甘肃贝母组织培养的外植体及灭菌方法,探究外植体种类、茎段大小、培养温度、激素组合、培养方式等对小鳞茎诱导和增殖的影响。结果:休眠芽的最佳灭菌方式为75%乙醇灭菌30 s,0.1%升汞灭菌1 min,无菌水冲洗3~5次。休眠芽为外植体时培养效果最好,其次为茎段和小鳞叶,叶片效果较差。茎段大小对甘肃贝母组培小鳞茎诱导影响不大。固体培养基更适宜直接诱导小鳞茎,培养基配方为:1/2 MS+6-BA 0.5 mg/L+NAA 0.1 mg/L或1/2 MS+6-BA 1.0 mg/L+NAA 1.0 mg/L,蔗糖3%、琼脂0.7%,pH 5.8。一定的变温培养有利于其鳞茎增殖。适宜的生根培养基为1/2 MS+NAA 0.1 mg/L+IAA 0.5 mg/L+1.5%蔗糖+0.7%琼脂,pH 5.8。结论:建立了甘肃贝母离体培养小鳞茎直接发生技术体系,可为甘肃贝母种质保存及工厂化育苗提供技术支撑。Objective:To establish the direct organogenesis technology system of small bulbs in tissue cultivation of Fritillaria przewalskii Maxim.Methods:A one-way experimental design was used to screen the suitable explants and sterilization methods for the tissue culture of F.przewalskii Maxim.The effects of explant type,stem size,culture temperature,hormone combination and culture method on the induction and proliferation of small bulbs were investigated.Results:The best sterilization method for dormant buds was sterilizing with 75% ethanol for 30 seconds,0.1% HgCl2 for 1 minute,and rinsing with sterile water for 3-5 times.Dormant buds were the best explants,followed by the stem segments and small scaly leaves,while the effect of leaves was poor.The size of stem segments had little effect on the induction of small bulbs in F.przewalskii Maxim.The solid medium was more suitable for direct induction of small bulbs,with a medium formulation of 1/2 MS + 6-BA 0.5 mg/L + NAA 0.1 mg/L or 1/2 MS + 6-BA 1.0 mg/L +NAA 1.0 mg/L,sucrose 3%,agar 0.7%,pH 5.8.A certain amount of variable temperature culture was beneficial to its bulb proliferation.The suitable rooting medium was 1/2 MS + NAA 0.1 mg/L + IAA 0.5 mg/L +1.5% sucrose + 0.7% agar,pH 5.8.Conclusion:The direct organogenesis technology system of small bulbs in isolated culture of F.przewalskii Maxim.was established,which could provide technical support for the conservation of germplasm and industrial seedling rearing of F.przewalskii Maxim.

关 键 词：甘肃贝母 离体培养 外植体 小鳞茎增殖 

分 类 号：R284.2[医药卫生—中药学]