基于SSR分子标记的茶树新品种‘戚家美铭’的亲缘关系分析  

Genetic Relationship Analysis of a New Tea Variety ‘Qijiameiming’ Based on SSR Molecular Markers

作　　者：刘国豪 杨倩 余速航 夏修新 吴双 倪穗[1] Liu Guohao;Yang Qian;Yu Suhang;Xia Xiuxin;Wu Shuang;Ni Sui(School of Marine Sciences,Ningbo University,Ningbo 315211,China)

机构地区：[1]宁波大学海洋学院,浙江宁波315211

出　　处：《中国野生植物资源》2023年第3期67-73,共7页Chinese Wild Plant Resources

基　　金：宁波市公益性科技计划项目(2021S008)。

摘　　要：目的:探明茶树新品种‘戚家美铭’的亲缘关系。方法:以‘戚家美铭’和同其栽种于同一茶园的两个茶树品种‘鸠坑’和‘乌牛早’,以及叶片形状类似的近似品种‘嘉茗一号’作为研究对象,利用21对SSR引物对其进行聚类分析。结果:21对引物共扩增出102个条带,每个SSR位点的观测等位基因数为2~7个,平均值为4.857 1,多态性达到100%,Shannon多态性信息指数范围在1.906 2~0.693 1,平均值为1.449 5,‘戚家美铭’与‘鸠坑’的遗传距离为1.343 7、遗传相似系数为0.260 9。结论:确定‘戚家美铭’是‘鸠坑’的自然杂交后代。Objective:To explore the genetic relationship of the new tea variety 'Qijiameiming'.Methods:Selecting two tea varieties 'Jiukeng' and 'Wuniuzao' planted in the same tea plantation as 'Qijiameiming' and a similar variety with similar leaf shape 'Jiaming No.1' as research objects and using 21 pairs of SSR primers to make cluster analysis.Results:The results showed that the 21 primer pairs amplified a total of 102 bands with 2 ~ 7 observed alleles per SSR locus,with an average of 4.857 1.The polymorphism reached 100% and the Shannon polymorphism information index ranged from 1.906 2 to 0.693 1with an average of 1.449 5.The genetic distance between 'Qijiameiming' and 'Jiukeng' was 1.343 7 and the genetic similarity coefficient was 0.260 9.Conclusion:It is determined that 'Qijiameiming' is the natural hybrid offspring of 'Jiukeng'.

关 键 词：茶树 新品种 SSR分子标记技术 亲缘关系 

分 类 号：S338[农业科学—作物遗传育种]