基于差异基因表达谱的Microbacterium sp.XT11降解黄原胶潜能挖掘  

Excavation of Xanthan Degradable Potential of Microbacterium sp.XT 11 Based on Differential Gene Expression Spectrum

作　　者：袁悦 王雪妍 李宪臻[1] 杨帆[1] YUAN Yue;WANG Xue-yan;LI Xian-zhen;YANG Fan(Schl.of Bio-Engin.,Dalian Polytech.Uni.,Dalian 116034)

机构地区：[1]大连工业大学生物工程学院,辽宁大连116034

出　　处：《微生物学杂志》2023年第1期20-31,共12页Journal of Microbiology

基　　金：国家自然科学基金项目(31671796);国家自然科学基金项目(32072160);辽宁省教育厅科学技术研究项目(J2020041);辽宁省教育厅科学技术研究项目(2016J002);。

摘　　要：为挖掘微杆菌(Microbacterium sp.)XT11在黄原胶降解过程中起关键作用的功能基因,预测黄原胶降解通路,利用转录组测序技术对该菌株在不同碳源培养条件下的转录本进行测序,对差异基因进行功能富集分析。结果表明,菌株XT11以葡萄糖为对照组,以黄原胶为碳源时可获得上调差异基因213个。显著上调的基因主要富集在聚糖降解、淀粉和蔗糖代谢途径、ABC转运、苯丙氨酸代谢、丙酮酸代谢五个KEGG途径。碳水化合物活性酶(Carbohydrate-active enzymes,CAZymes)功能注释表明,位于同一基因簇上的4个CAZymes基因和黄原胶降解直接相关,其余的CAZymes基因具有潜在的黄原胶降解活性。此外,预测到磷酸转移酶系统(phosphotransferase system,PTS)和ABC转运途径(ABC transporters)参与了胞外黄原胶降解中间产物的跨膜转运。挖掘了菌株XT11中黄原胶降解过程中的功能基因,并阐述了菌株XT11的黄原胶降解通路。In order to explore the critical functional genes in xanthan degradation and predict the xanthan degradation pathway in strain Microbacterium sp.XT11,the transcripts of XT11 cultured in different carbon sources was sequenced by RNA-Seq technology,and the differential genes were subjected to functional enrichment analysis in this study.The results showed that the strain XT11 using glucose taken as control group,when xanthan gum was used as a carbon source,213 genes were found to be up-regulated.And significantly up-regulated genes were mainly enriched in 5 KEGG pathways,including other glycan degradation,starch and sucrose metabolism pathway,ABC transporting protein,phenylalanine metabolism,and pyruvate metabolism.The functional annotation of carbohydrate-active enzymes(CAZymes)showed that four CAZymes genes were located in the same cluster and directly related to xanthan degradation,and the remaining CAZymes genes have potential xanthan degradable activity.Moreover,the phospho-transferase system(PTS)and ABC transporting protein system played an important role in the transmembrane transport of degradation intermediates of extracellular xanthan gum were prognosticated.In this study,the functional genes that play a crucial role in xanthan degradation of strain XT11 were explored,and the xanthan degradation pathway was expatiated.

关 键 词：Microbacterium sp.XT11 黄原胶降解 转录组 差异表达基因 富集分析 

分 类 号：Q936[生物学—微生物学]