Genome-wide identification and characterization of ACBP gene family in Populus reveal salinity alkali-responsive profiles  

作　　者：Yu Chang Xinru Xu Hongxia Zheng Hao Xie Bo Li Sixue Chen Ying Li Shaojun Dai 

机构地区：[1]Key Laboratory of Saline-Alkali Vegetation Ecology Restoration(Northeast Forestry University),Ministry of Education,College of Life Sciences,Northeast Forestry University,Harbin 150040,People’s Republic of China [2]Development Center of Plant Germplasm Resources,College of Life Sciences,Shanghai Normal University,Shanghai 200234,People’s Republic of China [3]Department of Biology,Genetics Institute,Plant Molecular and Cellular Biology Program,Interdisciplinary Center for Biotechnology Research,University of Florida,Gainesville,Florida,USA

出　　处：《Journal of Forestry Research》2023年第2期481-496,共16页林业研究（英文版）

基　　金：supported by grants from the Fundamental Research Funds for the Central Universities(Nos.2572018B03);the College Students Innovations Special Project funded by NEFU(No.202010225173);the Fundamental Research Funds for the Central Universities(Nos.2572019CT03);the Natural Science Foundation of Heilongjiang Province(No.ZD2019C003)。

摘　　要：Acyl-CoA-binding proteins(ACBPs)are important for the transport of acyl groups for macro molecular biosynthesis involved in plant growth,development,and diverse stress(e.g.,cold,drought,salinity,and heavy metals)responses.Here,we report the phylogeny and characteristics of the ACBP family in the woody plant Populus trichocarpa.Eight genes encoding ACBP proteins were identified,and they are distributed on eight chromosomes in P.trichocarpa.These PtACBP genes were divided into four subgroups according to gene structure,conserved motifs and phylogenetic relationship.Promoter analysis revealed that cis-elements were related to stress response,phytohormone response,and physical and reproductive growth regulation.Expression levels of PtACBP genes varied among different organs,with the highest expression in leaves and the lowest in stems.Quantitative real-time PCR(qRT-PCR)analysis showed that under salinity-alkali stresses(i.e.,200 mM NaCl,75 mM Na2CO3,and 100 mM NaHCO3),four(PtACBP1,PtACBP3,PtACBP4 and PtACBP8)of eight PtACBP genes were significantly induced in roots and leaves.These data provide a comprehensive analysis of the ACBPs family in P.trichocarpa,which could be useful for gene function analyses.

关 键 词：Acyl-CoA-binding proteins(ACBPs) Gene structure Expression pattern Stress response Populus trichocarpa 

分 类 号：S792.11[农业科学—林木遗传育种]