低温胁迫下单瓣黄刺玫抗氧化酶活性及相关基因表达  

Antioxidant Enzyme Activity and Related Gene Expression of Rosa xanthina f.normalis in Response to Cold Stress

作　　者：庄得凤 王成[1] 包金花[1] 杜晓燕 Zhuang Defeng;Wang Cheng;Bao Jinhua;Du Xiaoyan(Inner Mongolia Minzu University,Tongliao 028000,P.R.China)

机构地区：[1]内蒙古民族大学,通辽028000

出　　处：《东北林业大学学报》2023年第4期89-92,107,共5页Journal of Northeast Forestry University

基　　金：内蒙古民族大学博士启动基金项目(BS561)。

摘　　要：为了研究蔷薇属植物抵御寒冷气候抗氧化酶系统的响应机制,以2年生单瓣黄刺玫扦插植株为试验材料,通过人工模拟低温处理,以23℃为对照,在4、-5、-10、-15、-20℃胁迫下,分析了抗氧化酶的变化及相关基因表达情况。结果表明:叶片丙二醛(MDA)质量摩尔浓度先上升后下降;超氧化物歧化酶(SOD)活性呈波浪状升高,过氧化氢酶(POD)活性表现出上升下降再上升的趋势。以23℃为对照,对低温处理4℃和-20℃的叶片进行了转录组测序分析,共检测到6类29个抗氧化酶基因显著表达,在4℃时,有9个基因RxSOD1、RxSOD2、RxPOD42、RxPOD17、RxGST1、RxGST2、RxGPX6、RxAPX2、RxAPX3显著上调表达,-20℃时,只有2个基因RxSOD5、RxPOD47显著上调表达。To elucidate the antioxidant enzyme response mechanism of Rosa under low temperature stress,biennial Rosa xanthina f.normalis was used as materials in this study,simulating low temperature treatment by artificial condition under 4,-5,-10,-15 and-20℃stress with 23℃as a control,we analyzed antioxidant enzyme activity and related gene expression.The results indicated that MDA increased first and then decreased,SOD activity increased wavy,POD activity showed a trend with an upward and downward,and then upward.Transcriptome sequencing was performed under 4 and-20℃stress with 23℃as a control,a total of 6 classes including 29 antioxidant enzyme genes were significantly expressed,9 genes(RxSOD1,RxSOD2,RxPOD42,RxPOD17,RxGST1,RxGST2,RxGPX6,RxAPX2,RxAPX3)showed upregulated at 4℃,2 genes(RxSOD5 and RxPOD47)showed upregulated at-20℃.

关 键 词：单瓣黄刺玫 抗氧化酶 基因表达 低温胁迫 

分 类 号：S685.12[农业科学—观赏园艺]