华南地区鲍曼不动杆菌流行株的毒力变迁研究  

The virulence of the prevalent clone ST457 of Acinetobacter baumannii in Southern China

作　　者：唐翔 曾军[1] 卓超 杨智[1] 苏伟[1] TANG Xiang;ZENG Jun;ZHUO Chao;YANG Zhi;SU Wei(Intensive Care Unit,Guangzhou First People s Hospital,Guangzhou 510180,China;Guangzhou Institute of Respiratory Health,Guangzhou 510120,China)

机构地区：[1]广州市第一人民医院重症医学科,广州510180 [2]广州呼吸疾病健康研究院,广州510120

出　　处：《广州医药》2023年第3期91-96,112,共7页Guangzhou Medical Journal

基　　金：广州市一般引导项目(20201A011011);广州市第一人民医院红棉计划(Q2019013)。

摘　　要：目的探讨我国南方地区鲍曼不动杆菌流行株ST457毒力进化方向。方法收集南方地区5家大型三甲医院2012年—2015年血和/或痰标本来源的鲍曼不动杆菌菌株,运用不同毒力实验,比较ST457与其他CC92流行克隆株与非流行克隆株的毒力差异。结果ST457感染患者早期死亡率高;ST457菌株平均产生物膜能力低于ST195和ST208;83.3%的鲍曼不动杆菌临床分离菌株在血清的存活率大于60%,其中43.3%的菌株可在血清内快速繁殖,不同ST型组间、菌血症组和非菌血症组间无差异;不同ST型菌株竞争后,ST457未展现出竞争优势。结论与传统CC92流行株相比,ST457毒力表型与传统的CC92流行株类似,提示CC92克隆株共进化能力。部分流行株短时间内在血清中快速繁殖,提示其条件致病后潜在的血流感染的能力。Objective To investigate the virulence of the prevalent clone ST457 of Acinetobacter baumannii in Southern China.Methods Isolates from sputum and/or blood samples from 2012 to 2015 in 5 large differences between clonal complex 92(CC92)and non-CC92 were estimated by biofilm assay,competition assay and serum assay.Results Patients with ST457 infection had high early mortality.The average biofilm production of ST457 was significantly lower than that of ST195 and ST208.In serum assay,83.3%isolates survived in the serum with over 60%survival rate,of which 43.3%strains reproduced in the serum,with no difference between STs or between bacteremia and non-bacteremia group.After competing with different ST strains,ST457 shows no competitive advantage.Conclusions ST457 had a similar virulence phenotype with the traditional prevalent CC92 clone,suggesting the co-evolutionary ability for CC92 clone.Some clinical isolates were survived in human serum,suggesting the potential capacity of bloodstream infection.

关 键 词：鲍曼不动杆菌 ST457 毒力 

分 类 号：R446.5[医药卫生—诊断学]