菟丝草药材的抗炎活性评价及GC-MS成分分析  

Evaluation of Anti-inflammatory Activities of Cuscuta chinensis Herb and Chemical Analysis by GC-MS

作　　者：朱庆梅 戴乐 赵亚男 张友有 阿迪拉·阿布都热西提 Zhu Qingmei;Dai Le;Zhao Yanan;Zhang Youyou;Adila·Abudurexiti(College of Chemistry and Environmental Sciences,Laboratory of Xinjiang Native Medicinal and Edible Plant Resources Chemistry,Kashi University,Kashi 844006,China)

机构地区：[1]喀什大学化学与环境科学学院,新疆特色药食用植物资源化学实验室,新疆喀什844006

出　　处：《南开大学学报（自然科学版）》2023年第1期89-95,共7页Acta Scientiarum Naturalium Universitatis Nankaiensis

基　　金：新疆维吾尔自治区重点实验室开放课题(2020D04023);喀什大学研究生科研创新项目(2021KHY033)。

摘　　要：通过测定菟丝草药材对15-LOX、COX-2酶的抑制率来评价其体外抗炎活性,菟丝草药材各溶剂提取物对COX-2酶有较高的抑制率,其中石油醚提取物的活性最高;而对15-LOX酶的抑制率均比较低.同时采用GC-MS技术对其脂溶性部位进行成分分析,并根据峰面积归一化法对各组分进行定量分析.通过分析,共鉴定出酯类、脂肪酸、甾类等47种成分.结果显示,菟丝草药材有一定的抗炎活性,石油醚提取物活性最强,脂溶性提取物中甾类及脂肪酸成分含量较高.The anti-inflammatory activity of Cuscuta chinensis herbs was evaluated in vitro by measuring the inhibition rates to 15-LOX and COX-2 enzymes. The inhibition rate of 15-LOX enzyme in all parts of Cuscuta chinensis herb was relatively low, and the inhibition rate of COX-2 enzyme was higher,among which the petroleum ether part was the highest. At the same time, GC-MS was used to analyze the components of the fat soluble parts, and the quantitative analysis was carried out according to the peak area normalization method. A total of 47 components such as esters, fatty acids and steroids were identified by GC-MS. The results show that Cuscuta chinensis has certain anti-inflammatory activities and it has higher content of steroids and fatty acids in its fat-soluble parts.

关 键 词：菟丝草 抗炎 15-LOX COX-2 GC-MS分析 

分 类 号：R284.1[医药卫生—中药学]