L-苯丙氨酸的氨基功能化γ-Fe_(2)O_(3)磁固相萃取  

Magnetic Solid Phase Extraction of L-phenylalanine by Amino-functionalized γ-Fe_(2)O_(3)

作　　者：秦铭杉 朱欣怡 韦冬微 宋淇淇 金天明 李存 杨琳燕 Qin Mingshan;Zhu Xinyi;Wei Dongwei;Song Qiqi;Jin Tianming;Li Cun;Yang Linyan(Tianjin Key Laboratory of Agricultural Animal Breeding and Healthy Husbandry,College of Animal Science and Veterinary Medicine,Tianjin Agricultural University,Tianjin 300392,China;Tianjin Academy of Agricultural Sciences,Tianjin,300192,China)

机构地区：[1]天津农学院动物科学与动物医学学院,天津市农业动物繁育与健康养殖重点实验室,天津300392 [2]天津市农科院,天津300192

出　　处：《南开大学学报（自然科学版）》2023年第1期102-111,共10页Acta Scientiarum Naturalium Universitatis Nankaiensis

基　　金：Supported by Outstanding Research Achievement Award Project of Tianjin Agricultural University;the Open Topic of Guangxi Key Laboratory for the Chemistry and Molecular Engineering of Medicinal Resources (CMEMR2016-B12);the National Natural Science Foundation of China (21875117, 31572492);the Municipal-level Innovative and Entrepreneurial Training Plan for College Students (202210061109);the Veterinary Bitechnology Scientific Research Innovation Team of Tianjin,China (TD13-5091);the Veterinary“Innovative Talent People of Young and Middle Age Key Members Project”of Tianjin;the Natural Science Foundation of Tianjin,China (18JCYBJC30100, 19JCQNJC13700)。

摘　　要：通过不同的修饰方法制备了以γ-Fe_(2)O_(3)为核的四种氨基化的磁性纳米材料分别为γ-Fe_(2)O_(3)@SiO2-cysteamine, γ-Fe_(2)O_(3)@SiO2-PEI, γ-Fe_(2)O_(3)@SiO2-G1.0, γ-Fe_(2)O_(3)@SiO2-APTMS.在吸附时间为0.5, 1, 2,4, 8 h, p H=3, 5, 7, 9, 11的条件下,通过磁固相萃取的方法吸附浓度为2.5×10^(-6) mol/L的苯丙氨酸,随后用荧光分光光度计检测上清液的荧光强度,荧光强度越低说明纳米材料的吸附效果越优秀.通过试验后发现γ-Fe_(2)O_(3)@SiO2-G1.0磁性纳米粒子在p H=5,吸附时间为8 h的条件下的吸附性最好. γ-Fe_(2)O_(3)@SiO2-PEI磁性纳米粒子在pH=3,吸附时间为0.5 h条件下的吸附性略逊于上者,但该材料的制备过程简易,吸附氨基酸所需时间更短.因此功能化的γ-Fe_(2)O_(3)磁性纳米材料用于固相萃取氨基酸具有一定的研究前景.Four aminogenized nanoparticles were prepared as follows: γ-Fe_(2)O_(3)@SiO2-cysteamine, γ-Fe_(2)O_(3)@SiO2-APTMS, γ-Fe_(2)O_(3)@SiO2-PEI, γ-Fe_(2)O_(3)@SiO2-G1.0. Magnetic solid phase extraction experiments of L-phenylalanine have been carried out at a concentration of 2.5×10^(-6)mol/L, different extraction times and p H gradients. The lower fluorescence intensity of supernatant indicated the better extract effects.As for γ-Fe_(2)O_(3)@SiO2-G1.0 nanoparticles, the best adsorption was obtained at p H=5, while the adsorption time was 8 h. As for γ-Fe_(2)O_(3)@SiO2-PEI nanoparticles, less adsorption than above could be obtained at p H=3 and 0.5 h. γ-Fe_(2)O_(3)@SiO2-PEI nanoparticles took less time to extract L-phenylalanine. Functionalized γ-Fe_(2)O_(3)nanoparticles are promising for solid-phase extraction of L-phenylalanine.

关 键 词：Fe 表面化学 IR TEM XPS 吸附 

分 类 号：O658.2[理学—分析化学]