金属编码标签对许氏平鲉放流苗种的标记效果  

Marking Effect of Coded Wire Tag on Released Juveniles of Black Rockfish(Sebastes schlegelii)

作　　者：李影 徐永江[2] 崔爱君 姜燕[2] 王滨[2] 柳学周[2] 田云臣 LI Ying;XU Yongjiang;CUI Aijun;JIANG Yan;WANG Bin;LIU Xuezhou;TIAN Yunchen(College of Fisheries,Tianjin Agricultural University,Tianjin 300384,China;Yellow Sea Fisheries Research Institute,Chinese Academy of Fishery Sciences,Joint Laboratory for Open Sea Fishery Engineering,Pilot National Laboratory for Marine Science and Technology(Qingdao),Qingdao,Shandong 266071,China)

机构地区：[1]天津农学院水产学院,天津300384 [2]中国水产科学研究院黄海水产研究所、青岛海洋科学与技术试点国家实验室深蓝渔业工程联合实验室,山东青岛266071

出　　处：《渔业科学进展》2023年第2期40-49,共10页Progress in Fishery Sciences

基　　金：山东省支持青岛海洋科学与技术试点国家实验室重大科技专项(2018SDKJ0501-2);中国水产科学研究院基本科研业务费(TD47);中国水产科学研究院黄海水产研究所基本科研业务费(20603022020005);国家海洋水产种质资源库项目和财政部和农业农村部:国家现代农业产业技术体系共同资助。

摘　　要：利用金属编码标签(coded wire tag, CWT)对不同规格许氏平鲉(Sebastes schlegelii)放流苗种的不同部位(鳃部鄂弓提肌和背部肌肉)进行了标记实验,从标记苗种成活率、标签保持率、标记后苗种抗氧化酶活性及生长应激相关基因表达等方面评价了CWT的标记效果。结果显示,CWT标记30 d后,不同规格苗种成活率均为100%,小规格苗种鄂弓提肌标记组(SLM组)的实验鱼标签保持率为87%,大规格苗种鄂弓提肌标记组(LLM组)的实验鱼标签保持率为97%,苗种背部肌肉标记的标签保持率均为100%。CWT标记实验鱼特定生长率低于对照组。标记鱼暂养后,SLM组和大规格背部肌肉标记组(LDM)实验鱼肝脏过氧化氢酶(CAT)活性显著高于对照组,所有组实验鱼肝脏超氧化物歧化酶(SOD)活性均显著高于对照组。除SDM组外,其他实验组肝脏中IGF-1m RNA表达水平显著高于对照组,LDM组肝脏HSP70 m RNA表达水平最低。从存活率与标签保持率的角度考虑,CWT适宜于许氏平鲉苗种的标记放流,但CWT标记的苗种生长慢于对照组,且在免疫相关酶活性和生长及应激基因方面存在显著的表达差异,表明CWT标记操作对许氏平鲉苗种造成了一定的生理胁迫。因此,利用CWT标记的许氏平鲉苗种应有一个相对较长的室内暂养或野性驯化时间,有利于提高标记放流苗种成活率和放流后生长及回捕。Black rockfish(Sebastes schlegelii) is a member of the family Sebastidae—an ovoviviparous marine fish widely distributed in the coastal waters of China. This species has important commercial value and is also a delicious table fish. It often inhabits areas at the bottom of the sediment in rocky reefs with clear water quality. The species does not exhibit a long-distance migration habit or remarkable clustering. In recent years, sea ranching has developed rapidly in China. Black rockfish is a good candidate to promote sea ranching, because it does not exhibit a long migratory habit and is called a housekeeping fish. In recent decades, owing to the overfishing of wild resources and deterioration of the environment, the natural pools of black rockfish have decreased sharply. Thus, the national black rockfish release and breeding plan was implemented in 2006. Currently, millions of young black rockfish are released into the sea every year in China, which has played a positive role in promoting the conservation and restoration of natural resources. However, the release activity of marine organisms is random and under lax supervision. In recent years, various tagging technologies, including external markers, such as T-bar markers, and internal markers, such as otolith markers, have been developed to support the evaluation of the enhancement effect of released species. At present, otolith fluorescent labeling is the only reported marker technology that can be used for the proliferation and release of black rockfish.However, otolith staining has some problems, such as difficulty in detection and rapid fading, and it is not effective as an internal marker. Currently, there has been no efficient internal marker for black rockfish juveniles;thus, an effective marking technology must be established for evaluating the enhancement effect of black rockfish to support scientific release and natural resource maintenance. The coded wire tag(CWT) is a magnetized stainless-steel wire segment with a diameter of 0.25 mm. Several row

关 键 词：许氏平鲉 苗种 金属编码标签 标记效果 生理机制 

分 类 号：S965[农业科学—水产养殖]